Dujardin J C, Dujardin J P, Tibayrenc M, Timperman G, De Doncker S, Jacquet D, Arevalo J, Llanos-Cuentas A, Guerra H, Bermudez H
Laboratory of Protozoology, Institute of Tropical Medicine Prince Leopold, Antwerpen 1, Belgium.
Parasitology. 1995 Jan;110 ( Pt 1):21-30. doi: 10.1017/s0031182000081002.
A method for phenetic analysis of karyotype data has been developed for Leishmania populations. Measurement of size difference between chromosomes recognized by a given DNA probe in different isolates led to the formulation of a Chromosome Size Difference Index (CSDI). The method was applied to phenetic analysis of 4 sets of chromosomes--each set being recognized by a different probe--in 37 L. (Viannia) peruviana isolates sampled along a North-South transect through the Peruvian Andes and, in 11 L. (V.) braziliensis isolates from the Amazonian forest (Peru, Bolivia and Brazil). Karyotype variability was better accounted for by CSDI than by a method based on disjunctive encoding of karyotype data. CSDI evidenced the nature of relationships between L. braziliensis and L. peruviana and it provided a coherent picture of geographical and genomic differentiation among parasite populations. The latter did cluster according to their geographical origin. L. braziliensis was found karyotypically more homogeneous than L. peruviana. Within L. peruviana, Northern populations were closer to L. braziliensis than to Southern L. peruviana populations. The validity of karyotypic populations, or karyodemes, was sustained.
已开发出一种对利什曼原虫种群的核型数据进行表型分析的方法。测量不同分离株中由给定DNA探针识别的染色体之间的大小差异，从而制定了染色体大小差异指数（CSDI）。该方法应用于对沿着秘鲁安第斯山脉南北横断面采集的37株秘鲁维氏利什曼原虫分离株以及来自亚马逊森林（秘鲁、玻利维亚和巴西）的11株巴西维氏利什曼原虫分离株中的4组染色体（每组由不同探针识别）进行表型分析。与基于核型数据析取编码的方法相比，CSDI能更好地解释核型变异性。CSDI证明了巴西维氏利什曼原虫和秘鲁维氏利什曼原虫之间的关系性质，并提供了寄生虫种群地理和基因组分化的连贯图景。后者确实根据其地理起源聚类。发现巴西维氏利什曼原虫在核型上比秘鲁维氏利什曼原虫更具同质性。在秘鲁维氏利什曼原虫中，北部种群比南部种群更接近巴西维氏利什曼原虫。核型种群或核型类群的有效性得到了支持。
