Torrico M C, De Doncker S, Arevalo J, Le Ray D, Dujardin J C
Laboratory of Protozoology, Institute of Tropical Medicine Prince Leopold, Antwerpen, Belgium.
Acta Trop. 1999 Jan 15;72(1):99-110. doi: 10.1016/s0001-706x(98)00076-x.
In order to initiate studies on the phenotypic properties of hybrids vs. their putative parents, the in vitro growth behaviour of promastigotes was compared for 15 stocks characterised as Leishmania (Viannia) braziliensis, Leishmania (Viannia) peruviana and putative hybrids (isolated from the Eastern Andean valley of Huanuco, Peru). Five sets of three stocks, each set including a L.(V.)braziliensis, a L.(V.)peruviana and a putative hybrid, were constituted randomly and counted daily close to isolation from man (ten to 18 subcultures). Hybrids and L.(V.)peruviana presented similar growth characteristics, and they displayed a growth capacity (growth rate and cell density at stationary phase) significantly lower than the one of L.(V.)braziliensis. Following prolonged in vitro maintenance of one of the sets, the hybrid kept its lower growth capacity. The contrast between the difficulty to grow in vitro these putative hybrids, and their high isolation rate from natural populations is discussed.
为了开展关于杂种与其假定亲本的表型特性的研究，对15株被鉴定为巴西利什曼原虫（维氏亚属）、秘鲁利什曼原虫（维氏亚属）以及假定杂种（从秘鲁瓦努科的东安第斯山谷分离得到）的前鞭毛体的体外生长行为进行了比较。随机组建了五组，每组包含三株虫株，每组均包括一株巴西利什曼原虫（维氏亚属）、一株秘鲁利什曼原虫（维氏亚属）和一株假定杂种，并在接近从人体分离时（传代培养10至18次）每天进行计数。杂种和秘鲁利什曼原虫（维氏亚属）呈现出相似的生长特征，并且它们的生长能力（生长速率和稳定期细胞密度）显著低于巴西利什曼原虫（维氏亚属）。在对其中一组进行长时间体外传代培养后，杂种仍保持其较低的生长能力。文中讨论了这些假定杂种在体外培养困难与其在自然种群中高分离率之间的矛盾。
