Victoir K, Dujardin J C, de Doncker S, Barker D C, Arevalo J, Hamers R, Le Ray D
Instituut voor Tropische Geneeskunde Prince Leopold, Antwerpen, Belgium.
Parasitology. 1995 Sep;111 ( Pt 3):265-73. doi: 10.1017/s0031182000081828.
The genomic organization of gp63 genes in 4 and 7 isolates of Leishmania braziliensis and L. peruviana, respectively was studied by RFLP analysis with 3 restriction enzymes (Bgl I, Sal I and Apa I). Our results showed a marked polymorphism among isolates. Some characters were specific to L. braziliensis or to L. peruviana, and others specific to the respective biogeographical populations of L. peruviana. The average minimum copy number of gp63 genes was found to be higher in L. braziliensis (71) than in L. peruviana (46), suggesting that deletion of gp63 genes might be partially involved in the size decrease of the chromosome bearing gp63 genes, observed between those 2 species (from 700 to 610 kb). Our results may suggest the existence of at least 2 arrays of heterologous gp63 repeats, varying in relative copy number between L. braziliensis and L. peruviana, and among isolates of the latter species. Rearrangement of the gp63 genes was observed during long-term in vitro maintenance of a reference strain of L. braziliensis. These observations document the existence of a dynamic gp63 gene organization in Leishmania of the braziliensis complex.
分别用3种限制性内切酶（Bgl I、Sal I和Apa I）通过RFLP分析研究了巴西利什曼原虫4个分离株和秘鲁利什曼原虫7个分离株中gp63基因的基因组组织。我们的结果显示分离株之间存在明显的多态性。一些特征是巴西利什曼原虫或秘鲁利什曼原虫特有的，而其他特征是秘鲁利什曼原虫各自生物地理种群特有的。发现巴西利什曼原虫中gp63基因的平均最小拷贝数（71）高于秘鲁利什曼原虫（46），这表明gp63基因的缺失可能部分参与了在这两个物种之间观察到的携带gp63基因的染色体大小的减小（从700 kb到610 kb）。我们的结果可能表明存在至少2种异源gp63重复序列阵列，在巴西利什曼原虫和秘鲁利什曼原虫之间以及后一物种的分离株之间相对拷贝数有所不同。在巴西利什曼原虫参考菌株的长期体外培养过程中观察到了gp63基因的重排。这些观察结果证明了巴西利什曼原虫复合种利什曼原虫中存在动态的gp63基因组织。
