重组人克拉拉细胞10 kDa蛋白的结晶与表征

Crystallization and characterization of the recombinant human Clara cell 10-kDa protein.

作者信息

Matthews J H, Pattabiraman N, Ward K B, Mantile G, Miele L, Mukherjee A B

机构信息

Laboratory for the Structure of Matter, Naval Research Laboratory, Washington, DC 20375-5000.

出版信息

Proteins. 1994 Oct;20(2):191-6. doi: 10.1002/prot.340200209.

DOI:10.1002/prot.340200209

PMID:7846028

Abstract

Crystals of recombinant human Clara cell 10-kDa protein were grown both from ammonium sulfate and polyethylene glycol (PEG) solutions. Crystals grown from ammonium sulfate solution have been characterized by X-ray diffraction studies as monoclinic with the space group C2 and lattice constants a = 69.2 A, b = 83.0 A, c = 58.3 A, and beta = 99.7 degrees. The monoclinic crystals diffract to beyond 2.5 A. Some of the crystals grown from PEG were of a similar habit to those grown from ammonium sulfate, but others were triclinic with the space group P1 and cell constants a = 40.3 A, b = 46.3 A, c = 51.3 A, alpha = 117.7 degrees, beta = 102.3 degrees, and gamma = 71.4 degrees. These crystals diffract to beyond 3.2 A.

摘要

重组人克拉拉细胞10 kDa蛋白的晶体分别从硫酸铵溶液和聚乙二醇（PEG）溶液中生长出来。通过X射线衍射研究对从硫酸铵溶液中生长出的晶体进行了表征，其为单斜晶系，空间群为C2，晶格常数a = 69.2 Å，b = 83.0 Å，c = 58.3 Å，β = 99.7°。这些单斜晶体能衍射到2.5 Å以上。一些从PEG中生长出的晶体与从硫酸铵中生长出的晶体具有相似的晶习，但其他晶体为三斜晶系，空间群为P1，晶胞常数a = 40.3 Å，b = 46.3 Å，c = 51.3 Å，α = 117.7°，β = 102.3°，γ = 71.4°。这些晶体衍射到3.2 Å以上。