Lokesh B R, Kinsella J E
Lipids Research Group, University of California, Davis 95616.
Prostaglandins Leukot Essent Fatty Acids. 1994 Oct;51(4):235-9. doi: 10.1016/0952-3278(94)90185-6.
Mouse peritoneal macrophages were prelabelled with [3H]AA and activated ex vivo with phorbol myristate acetate (PMA) in the presence or absence of added n-3 polyunsaturated fatty acids (n-3 PUFA). The n-3 PUFA did not affect the release of [3H]AA from stimulated macrophages. Added AA, enhanced, in a dose-dependent manner, the amount of free [3H]AA in the medium after activating the cells with PMA. Both n-3 PUFA and AA were actively acylated into the lipids of PMA activated macrophages, but the n-3 PUFA did not compete with reacylation of AA. In unstimulated cells, in the absence of PMA, n-3 PUFA effectively competed with AA for acylation into membrane phospholipids. These studies suggest that distinct pools of AA exist in macrophages.
