Card G L, Jasuja R R, Gustafson G L
Stella Duncan Research Institute, Division of Biological Sciences, University of Montana, Missoula 59812.
J Leukoc Biol. 1994 Dec;56(6):723-8. doi: 10.1002/jlb.56.6.723.
The relative activities of lipoteichoic acid (LTA) from four Gram-positive bacteria were compared to different lipopolysaccharide (LPS) preparations for activation of arachidonic acid metabolism in mouse peritoneal macrophages. Total eicosanoid was determined in cultures labeled with [3H]-arachidonic acid. Prostaglandin E2 (PGE2) and leukotriene C4 (LTC4) were determined by EIA analysis. The relative potencies of the different preparations were: smooth LPS from Salmonella abortus > or = Re-LPS from Salmonella minnesota (R-595) > or = LTA from Streptococcus pyogenes approximately Streptococcus faecalis approximately Staphylococcus aureus > or = monophosphoryl lipid A derived from the Re-LPS >> LTA from Bacillus subtilis. Activation of eicosanoid release was inhibited by staurosporin for all of the amphiphiles tested. Treatment of the macrophage cultures with LTA from S. pyogenes, S. faecalis, and S. aureus, either in the presence or absence of indomethacin, desensitized the cells to eicosanoid release on subsequent challenge with LPS. The desensitized cells remained responsive to the phorbol ester phorbol myristate acetate. LPS from Gram-negative bacteria has immunostimulatory and endotoxic activities which result, in part, from the release of eicosanoids and other mediators from activated macrophages. The similarities in the patterns of cell activation by LPS and LTA suggest that lipoteichoic acids might contribute to the pathogenicities of Gram-positive bacteria.
比较了四种革兰氏阳性菌的脂磷壁酸（LTA）与不同脂多糖（LPS）制剂对小鼠腹腔巨噬细胞花生四烯酸代谢激活的相对活性。在用[3H] -花生四烯酸标记的培养物中测定总类二十烷酸。通过酶免疫分析测定前列腺素E2（PGE2）和白三烯C4（LTC4）。不同制剂的相对效力为：流产沙门氏菌的光滑LPS≥明尼苏达沙门氏菌（R - 595）的Re - LPS≥化脓性链球菌的LTA≈粪肠球菌≈金黄色葡萄球菌≥源自Re - LPS的单磷酰脂质A>>枯草芽孢杆菌的LTA。对于所有测试的两亲物，星形孢菌素抑制类二十烷酸释放的激活。用化脓性链球菌、粪肠球菌和金黄色葡萄球菌的LTA处理巨噬细胞培养物，无论是否存在吲哚美辛，在用LPS随后刺激时，细胞对类二十烷酸释放都变得不敏感。脱敏细胞仍然对佛波酯佛波醇肉豆蔻酸酯乙酸盐有反应。革兰氏阴性菌的LPS具有免疫刺激和内毒素活性，这部分是由于活化巨噬细胞释放类二十烷酸和其他介质所致。LPS和LTA激活细胞模式的相似性表明脂磷壁酸可能对革兰氏阳性菌的致病性有贡献。
