Xu G Y, Duanmu Z X, Yin Q Z
Laboratory of Neurobiology, Suzhou Medical College.
Sheng Li Xue Bao. 1994 Oct;46(5):427-34.
Rats with adjuvant arthritis were used as an animal model of pathological pain in this experiment and the nociceptive response of neurons in parafascicular nucleus (Pf) were recorded and intracerebroventricular (icv) injection of naloxone and atropine was adopted in order to investigate the effect of electroacupuncture (EA) and to analyze the neurotransmitters involved. The main results were as follows: (1) The nociceptive response of the majority of Pf neurons (29/34) in arthritic rats was significantly inhibited by EA at acupoints of "Zusanli" and "Sanyinjiao"; (2) The inhibitory effect of EA was reversed in 12/13 units by icv injection of naloxone (4 micrograms/10 microliters); (3) The inhibitory effect of EA was also reversed in 11/12 units by icv injection of atropine (5 micrograms/10 microliters) (4) The spontaneous unit discharge of Pf neurons in arthritic rats was increased after icv injection of naloxone or atropine. Experimental results suggest that EA could have an inhibitory effect on the nociceptive response of Pf neurons in arthritic rats, which might be mediated by opioid system and cholinergic system in the brain, and that opioid system and cholinergic system might have a tonic inhibitory effect on the spontaneous unit discharge of Pf neurons in arthritic rats.
本实验采用佐剂性关节炎大鼠作为病理性疼痛动物模型，记录束旁核（Pf）神经元的伤害性反应，并采用脑室内（icv）注射纳洛酮和阿托品，以研究电针（EA）的作用并分析其中涉及的神经递质。主要结果如下：（1）电针“足三里”和“三阴交”穴位可显著抑制大多数关节炎大鼠Pf神经元（29/34）的伤害性反应；（2）脑室内注射纳洛酮（4微克/10微升）可使12/13个单位的电针抑制作用逆转；（3）脑室内注射阿托品（5微克/10微升）也可使11/12个单位的电针抑制作用逆转；（4）脑室内注射纳洛酮或阿托品后，关节炎大鼠Pf神经元的自发放电单位增加。实验结果表明，电针对关节炎大鼠Pf神经元的伤害性反应具有抑制作用，这可能由脑内阿片系统和胆碱能系统介导，且阿片系统和胆碱能系统可能对关节炎大鼠Pf神经元的自发放电单位具有紧张性抑制作用。
