Taylor P J, Salm P, Norris R L, Ravenscroft P J, Pond S M
Department of Clinical Pharmacology, Princess Alexandra Hospital, Woolloongabba, Australia.
Ther Drug Monit. 1994 Oct;16(5):526-30. doi: 10.1097/00007691-199410000-00015.
Conflicting conclusions have been drawn from comparisons of high-performance liquid chromatography (HPLC) and the Abbott Diagnostics monoclonal fluorescence polarization immunoassay (mFPIA) for cyclosporin. The aim of this study was to compare whole blood cyclosporin A (CsA) concentrations measured by both mFPIA and HPLC in liver and heart transplant patients. One hundred and twenty-four liver and 62 heart transplant patient samples were assayed by both methods. Assay imprecision for both methods during the studies was < 7% over the range 150-800 micrograms/L. At an HPLC-determined concentration of 100 micrograms/L, mFPIA overestimated CsA by 60% (liver) and 77% (heart). At 300 micrograms/L, the overestimation was 40% (liver) and 45% (heart). On this basis, the mFPIA is not interchangeable with HPLC.
关于高效液相色谱法（HPLC）和雅培诊断公司的环孢素单克隆荧光偏振免疫分析法（mFPIA）的比较，已经得出了相互矛盾的结论。本研究的目的是比较肝移植和心脏移植患者中通过mFPIA和HPLC测得的全血环孢素A（CsA）浓度。采用这两种方法对124份肝移植患者样本和62份心脏移植患者样本进行了检测。在研究期间，两种方法在150 - 800微克/升范围内的检测不精密度均< 7%。在HPLC测定浓度为100微克/升时，mFPIA对CsA的高估在肝移植患者中为60%，在心脏移植患者中为77%。在300微克/升时，高估分别为40%（肝移植）和45%（心脏移植）。在此基础上，mFPIA与HPLC不可互换。
