Gulbis B, Van der Heijden J, van As H, Thiry P
Department of Clinical Chemistry, Hôpital Erasme-ULB, Brussels, Belgium.
J Pharm Biomed Anal. 1997 Apr;15(7):957-63. doi: 10.1016/s0731-7085(96)01938-3.
The specificity of two cyclosporin immunoassays were evaluated. Eleven patients were followed for the first four weeks after heart (n = 3) or liver (n = 8) transplantation. Cyclosporin A (CsA) monitoring was performed concomitantly by a monoclonal fluorescence polarization immunoassay (mFPIA) and enzyme-multiplied immunoassay technique (EMIT) during this period. For several patients, cyclosporin monitoring was also performed by high performance liquid chromatography (HPLC) or by polyclonal fluorescence polarization immunoassay (pFPIA). Liver function was assessed by follow-up of plasma total bilirubin, gamma-glutamyl transferase and alkaline phosphatase and renal function by plasma creatinine. All the patients presented episodes of impaired liver function. Higher CsA levels were found using mFPIA measurements as compared to the EMIT measurements (ratio mFPIA:EMIT (medium range) = 1.4 (1.0-2.3)). A higher degree of cross-reactivity of the antibody used in the mFPIA as compared to the EMIT was demonstrated by specific measurements of CsA and its primary metabolite, AM1, by HPLC.
对两种环孢素免疫测定法的特异性进行了评估。对11例心脏移植（n = 3）或肝脏移植（n = 8）后的患者进行了为期四周的随访。在此期间，通过单克隆荧光偏振免疫测定法（mFPIA）和酶放大免疫测定技术（EMIT）同时进行环孢素A（CsA）监测。对于部分患者，还通过高效液相色谱法（HPLC）或多克隆荧光偏振免疫测定法（pFPIA）进行环孢素监测。通过随访血浆总胆红素、γ-谷氨酰转移酶和碱性磷酸酶评估肝功能，通过血浆肌酐评估肾功能。所有患者均出现肝功能受损情况。与EMIT测量结果相比，使用mFPIA测量时发现CsA水平更高（mFPIA与EMIT的比值（中等范围）= 1.4（1.0 - 2.3））。通过HPLC对CsA及其主要代谢产物AM1进行特异性测量，证明mFPIA中使用的抗体与EMIT相比具有更高程度的交叉反应性。
