Matiakis A, Konstantinidis A, Trigonidis G, Papanayotou P
Department of Oral Medicine and Pathology, School of Dentistry, Aristotle University of Thessaloniki, Greece.
Ann Dent. 1994 Winter;53(2):16-20.
The purpose of this study is to determine the role of the time of application of the promoter (in this case, mechanical irritation) in two-stage chemically induced oral carcinogenesis. One hundred fifty-five male rats were divided into seven groups. Group 1, 2 and 3 each received two intraperitoneal injections of the carcinogen N-nitroso-N-methylurea (NMU): 50 mg/Kg BW. Mechanical irritation was applied on the left buccal mucosa, 7 days before the first injection of NMU (group 1), 16 days (group 2), and 80 days (group 3) after the carcinogenic administration. Animals of groups 4, 5, 6 and 7 served as controls. Only group 1 and 2 animals developed tumors in the irritated buccal mucosa. From the results of this study we conclude that the delay of the application of the promoter does not lead to tumor formation. This could be explained by the repair of the carcinogenic lesion at the cellular level.
本研究的目的是确定启动子（在本研究中为机械刺激）的应用时间在两阶段化学诱导口腔癌发生过程中的作用。155只雄性大鼠被分为7组。第1、2和3组分别接受两次腹腔注射致癌物N-亚硝基-N-甲基脲（NMU），剂量为50mg/Kg体重。在第一次注射NMU前7天（第1组）、致癌给药后16天（第2组）和80天（第3组）对左侧颊黏膜施加机械刺激。第4、5、6和7组动物作为对照。只有第1组和第2组动物在受刺激的颊黏膜中发生了肿瘤。从本研究结果我们得出结论，启动子应用的延迟不会导致肿瘤形成。这可以通过细胞水平上致癌损伤的修复来解释。
