Zelada-Hedman M, Werer G, Collins P, Bäckdahl M, Perez I, Franco S, Jimenez J, Cruz J, Torroella M, Nordenskjöld M
National Institute of Oncology and Radiobiology, Havana City, Cuba.
Anticancer Res. 1994 Sep-Oct;14(5A):1679-88.
The epidermal growth factor receptor and its ligands have been implicated as being involved in normal mammary development and breast cancer genesis. Northern blotting was used to assay the mRNA levels of the epidermal growth factor receptor and three of its ligands: the epidermal growth factor, the transforming growth factor alpha and the Amphiregulin in 16 primary carcinomas, 2 metastases and 5 fibroadenomas. In addition, the mRNA levels of the other members of the epidermal growth factor receptor family, erbB2 and erbB3 were also analysed. We found limited expression in the breast carcinomas while all the fibroadenomas showed expression at high levels. Therefore we suggest that the epidermal growth factor receptor plays an important role in the development of fibroadenomas. The erbB2 and erbB3 were more strongly expressed than the epidermal growth factor receptor in the primary carcinomas. This suggests that they could be of importance in breast carcinogenesis.
表皮生长因子受体及其配体被认为参与正常乳腺发育和乳腺癌的发生。采用Northern印迹法检测了16例原发性癌、2例转移癌和5例纤维腺瘤中表皮生长因子受体及其三种配体（表皮生长因子、转化生长因子α和双调蛋白）的mRNA水平。此外，还分析了表皮生长因子受体家族其他成员erbB2和erbB3的mRNA水平。我们发现乳腺癌中的表达有限，而所有纤维腺瘤均呈高水平表达。因此，我们认为表皮生长因子受体在纤维腺瘤的发生中起重要作用。在原发性癌中，erbB2和erbB3的表达比表皮生长因子受体更强。这表明它们在乳腺癌发生中可能具有重要意义。
