Kim T H, Kim S H, Lee Y S, Choi S Y, Yoo S Y, Jang J J
Laboratory of Radiation Medicine, Korea Cancer Center Hospital, Seoul.
Anticancer Res. 1994 Sep-Oct;14(5A):1979-82.
We investigated the effect of potato extracts and 16,16-dimethyl prostaglandin E2 (DiPGE2) on the induction of glutathione S-transferase P-positive (GST-P+) altered hepatic foci in newborn Sprague-Dawley rats given single treatment with 60Co gamma irradiation and diethylnitrosamine (DEN) alone or in sequential combination. Intraperitoneal injection of 0.15 mumol/g body weight of DEN 1 hour after gamma radiation significantly increased the frequencies of GST-P+ hepatic foci compared to DEN or gamma radiation alone and DEN injection 1 hour before irradiation (p < 0.001). Potato extract was given at a dose of 2 mg/ml in drinking water for 3 weeks and DiPGE2 given at a dose of 10 micrograms/mouse 30 minutes before irradiation. Potato extracts and DiPGE2 decreased significantly the number (p < 0.001), area (p < 0.001) and Dmax (p < 0.05) of GST-P+ hepatic foci compared to the corresponding control. These results suggest that potato extracts and DiPGE2 have radio-protective potential and further studies for underlying mechanisms will be necessary.
我们研究了马铃薯提取物和16,16-二甲基前列腺素E2（DiPGE2）对新生斯普拉格-道利大鼠经60Coγ射线单独照射、二乙基亚硝胺（DEN）单独处理或二者先后联合处理后诱导谷胱甘肽S-转移酶P阳性（GST-P+）肝灶改变的影响。γ射线照射后1小时腹腔注射0.15μmol/g体重的DEN，与单独使用DEN或γ射线照射以及照射前1小时注射DEN相比，显著增加了GST-P+肝灶的频率（p < 0.001）。马铃薯提取物以2mg/ml的剂量加入饮用水中持续3周给予，DiPGE2在照射前30分钟以10μg/只小鼠的剂量给予。与相应对照组相比，马铃薯提取物和DiPGE2显著降低了GST-P+肝灶的数量（p < 0.001）、面积（p < 0.001）和最大直径（p < 0.05）。这些结果表明，马铃薯提取物和DiPGE2具有辐射防护潜力，有必要对其潜在机制进行进一步研究。
