Herrmann J E, Collins M F
J Immunol Methods. 1976;10(4):363-6. doi: 10.1016/0022-1759(76)90030-2.
Adsorption of 125I-labelled rabbit IgG to plastics (cellulose nitrate, polyallomer, polystyrene, polyvinyl) was primarily dependent on initial antibody concentration and, to a lesser extent, on time allowed for adsorption. The highest concentration tested (100 mug/ml) gave the highest quantities adsorbed after 18 h at room temperature. This concentration, however, gave the lowest percent adsorption (6.5 to 12.0%) of the initial amount. IgG concentration of 10 mug/ml resulted in 25.0 to 65.1% adsorption over the same time period; at 1 mug/ml, 47.0 to 96.6% of the initial amount was adsorbed. All of the plastics tested adsorbed IgG to approximately the same degree, with the exception of cellulose nitrate. This plastic adsorbed 32 to 49% less than the others, under maximal adsorption conditions.
125I标记的兔免疫球蛋白G（IgG）对塑料（硝酸纤维素、聚异丁烯、聚苯乙烯、聚乙烯）的吸附主要取决于初始抗体浓度，在较小程度上还取决于吸附时间。在室温下放置18小时后，测试的最高浓度（100微克/毫升）吸附量最高。然而，该浓度下的吸附百分率最低，仅为初始量的6.5%至12.0%。10微克/毫升的IgG浓度在相同时间段内的吸附率为25.0%至65.1%；1微克/毫升时，吸附量为初始量的47.0%至96.6%。除硝酸纤维素外，所有测试的塑料对IgG的吸附程度大致相同。在最大吸附条件下，这种塑料的吸附量比其他塑料少32%至49%。
