Yolken R H, Davis D, Winkelstein J, Russell H, Sippel J E
J Clin Microbiol. 1984 Oct;20(4):802-5. doi: 10.1128/jcm.20.4.802-805.1984.
A solid-phase immunoassay utilizing horse antiserum against the C polysaccharide of Streptococcus pneumoniae and biotinylated rabbit antibodies to type-specific pneumococcal polysaccharides was developed to detect pneumococcal antigens in human body fluids and in broth cultures. Pneumococcal antigen could be detected in broth cultures of serotypes of S. pneumoniae containing as little as 10(2) to 10(3) organisms per ml. The assay system detected pneumococcal antigen in all 25 cerebrospinal fluid specimens obtained from patients with documented pneumococcal meningitis. There were no positive reactions noted in specimens from patients infected with Neisseria meningitidis group A or from patients without evidence of bacterial infection. The solid-phase enzyme immunoassay utilizing these reagents is a sensitive and specific assay for the immunodetection of a wide range of pneumococcal antigens.
利用抗肺炎链球菌C多糖的马抗血清和针对特定血清型肺炎球菌多糖的生物素化兔抗体开发了一种固相免疫测定法，用于检测人体体液和肉汤培养物中的肺炎球菌抗原。在每毫升含有低至10²至10³个菌体的肺炎链球菌血清型肉汤培养物中可检测到肺炎球菌抗原。该检测系统在从有记录的肺炎球菌脑膜炎患者获得的所有25份脑脊液标本中均检测到了肺炎球菌抗原。在感染A群脑膜炎奈瑟菌的患者标本或无细菌感染证据的患者标本中未发现阳性反应。利用这些试剂的固相酶免疫测定法是一种用于广泛免疫检测肺炎球菌抗原的灵敏且特异的检测方法。
