Li M, White E H
Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218.
Bioconjug Chem. 1994 Sep-Oct;5(5):454-8. doi: 10.1021/bc00029a012.
Two fluorogenic N-nitrosoamides, N-nitroso-N-((7-methoxycoumarin-4-yl)methyl)-N'-isobutyrylalaninamide (6a) and N-nitroso-N-((6-methoxyquinolin-2-yl)methyl)-N'-isobutyrylalani namide (6b), were synthesized. Both N-nitrosoamides inhibited alpha-chymotrypsin irreversibly; they show promise as labeling reagents for the active sites of chymotrypsin-like proteases.
合成了两种荧光N-亚硝基酰胺，即N-亚硝基-N-((7-甲氧基香豆素-4-基)甲基)-N'-异丁酰丙氨酰胺(6a)和N-亚硝基-N-((6-甲氧基喹啉-2-基)甲基)-N'-异丁酰丙氨酰胺(6b)。这两种N-亚硝基酰胺均不可逆地抑制α-胰凝乳蛋白酶；它们有望作为类胰凝乳蛋白酶活性位点的标记试剂。
