Elsner H I, Mouritsen S
M&E, Lersø, Copenhagen, Denmark.
Bioconjug Chem. 1994 Sep-Oct;5(5):463-7. doi: 10.1021/bc00029a014.
The ability of compounds to adsorb passively to hydrophobic polymer surfaces composed of, e.g., polystyrene generally is restricted to limited types of molecules such as proteins. Some proteins, many peptides, polysaccharides, oligonucleotides, and small molecules as well as pro- and eucaryotic cells cannot adsorb directly to such surfaces. Also, solid phase adsorbed antigens, antibodies, or gene probes may not be recognized by its corresponding ligand due to denaturation or steric hindrance of the molecular tertiary structure. Covalent binding, on the other hand, orientates all immobilized compounds in a defined way on the solid phase, thereby exposing the interacting sites on the enzymes, antibodies, gene probes, etc. Here we describe a method for modifying a polymer surface by contacting the polymer with derivatives of psoralen under irradiation with long-wavelength UV light. The psoralen derivatives were immobilized covalently on the polymer surface by this process. The psoralen molecules was conjugated to appropriate chemical linkers, incubated in aqueous solutions, and irradiated with UV light. This resulted in solid phase introduction of functional groups such as, e.g., amino groups on the polystyrene surface. The functional groups could subsequently be used for immobilization of biomolecules using conventional cross-linker technology. The method only involved premodification of the psoralens to be immobilized whereas no pretreatment of the polymer was required. Psoralen modified microtiter plates seems to have future application for the development of solid phase hybridization and immunoassays.
化合物被动吸附到由例如聚苯乙烯组成的疏水聚合物表面的能力通常仅限于有限类型的分子，如蛋白质。一些蛋白质、许多肽、多糖、寡核苷酸、小分子以及原核和真核细胞不能直接吸附到此类表面。此外，由于分子三级结构的变性或空间位阻，固相吸附的抗原、抗体或基因探针可能无法被其相应的配体识别。另一方面，共价结合以确定的方式将所有固定化化合物定向在固相上，从而暴露酶、抗体、基因探针等上的相互作用位点。在此，我们描述了一种通过使聚合物与补骨脂素衍生物在长波长紫外光照射下接触来修饰聚合物表面的方法。通过该过程，补骨脂素衍生物共价固定在聚合物表面。补骨脂素分子与合适的化学连接体偶联，在水溶液中孵育，并用紫外光照射。这导致在聚苯乙烯表面固相引入官能团，例如氨基。随后可以使用传统的交联剂技术将官能团用于固定生物分子。该方法仅涉及对要固定的补骨脂素进行预修饰，而无需对聚合物进行预处理。补骨脂素修饰的微量滴定板似乎在固相杂交和免疫测定的开发中有未来应用。
