Maeda M, Nishimura C, Umeno D, Takagi M
Department of Chemical Science and Technology, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
Bioconjug Chem. 1994 Nov-Dec;5(6):527-31. doi: 10.1021/bc00030a007.
We have synthesized a vinyl monomer having a psoralen moiety, which can form a photoadduct with double-helical DNA. The monomer 1 was proved to have an ability to crosslink DNA double strands through a photochemical reaction when irradiated by UV light. The resulting DNA having vinyl groups was copolymerized with a comonomer such as acrylamide and N-isopropylacrylamide to give rise to a DNA-vinyl polymer conjugate. A conjugation based on covalent bondings was verified by using gel electrophoresis; the conjugation efficiency was found to be dependent upon concentration of the monomer which had been used in the antecedent photochemical reaction. This monomer will be a useful tool when anchoring double-helical DNA on polymeric materials for separating and sensing DNA-binding substances.
我们合成了一种带有补骨脂素部分的乙烯基单体，它能与双螺旋DNA形成光加合物。单体1经证明在紫外线照射下能够通过光化学反应交联DNA双链。所得带有乙烯基的DNA与诸如丙烯酰胺和N-异丙基丙烯酰胺等共聚单体进行共聚，从而得到DNA-乙烯基聚合物共轭物。通过凝胶电泳验证了基于共价键的共轭作用；发现共轭效率取决于先前光化学反应中所使用单体的浓度。当将双螺旋DNA固定在用于分离和传感DNA结合物质的聚合材料上时，这种单体将是一种有用的工具。
