Buckingham R H
URA 1139 du CNRS, Institut de Biologie Physico-Chimique, Paris, France.
Biochimie. 1994;76(5):351-4. doi: 10.1016/0300-9084(94)90108-2.
The sequence around stop codons strongly affects termination efficiency and the probability of alternative events to termination such as frameshifting and stop codon readthrough. Where tRNA suppressors of nonsense codons are present, both the efficiency of suppression and of the termination process appear to be affected by stop codon context. Since context can affect suppressor tRNA function directly, an influence on sense codon translation or suppression might be expected, but has not yet been clearly demonstrated. Statistical analyses of coding sequences indicate non-random contexts for both stop and sense codons, and non-random occurrence of codon pairs. Highly expressed genes show clear preferences among stop codons and their contexts, whereas inefficient stop signals are exploited in a variety of recoding processes.
终止密码子周围的序列强烈影响终止效率以及诸如移码和终止密码子通读等终止替代事件的概率。在存在无义密码子的tRNA抑制子的情况下，抑制效率和终止过程似乎都受到终止密码子上下文的影响。由于上下文可以直接影响抑制性tRNA的功能，因此可能预期对有义密码子翻译或抑制有影响，但尚未得到明确证实。编码序列的统计分析表明，终止密码子和有义密码子的上下文都是非随机的，并且密码子对的出现也是非随机的。高表达基因在终止密码子及其上下文中表现出明显的偏好，而低效的终止信号则在各种重编码过程中被利用。
