Roy S K, Hughes J
Department of Obstetrics and Gynecology, Leland J. and Dorothy H. Olson Center for Women's Health, University of Nebraska Medical Center, Omaha 68198-4515.
Biol Reprod. 1994 Nov;51(5):821-30. doi: 10.1095/biolreprod51.5.821.
The differentiation of ovarian somatic cells into granulosa, interstitial, and thecal lineages, and the ovarian growth factor(s) potentially associated with the cytodifferentiation of granulosa cells during the perinatal period were investigated using cell type-specific protein markers. Ovaries collected from prenatal (Days 12-15 of pregnancy) and postnatal (Days 1-30) female hamsters were processed for immunohistochemical localization of transforming growth factor (TGF) beta (TGF beta 1, beta 2, and beta 3). Plasma levels of FSH, LH, progesterone, and estradiol-17 beta were detected by RIA. Prenatal hamster ovaries contained numerous mitotic oocytes and a few somatic cells. Only a fraction of somatic cells expressed barely detectable TGF beta 2 activity. Plasma FSH levels were quite detectable on postnatal Day 1 and increased gradually to reach a peak on Day 25, whereas LH did not increase until Day 12 and reached a plateau by postnatal Day 20. On postnatal Day 1, TGF beta 2 immunoreactivity was localized only in certain cells closely apposed to primordial oocytes. On postnatal Day 4, flattened, TGF beta 2-positive cells encircled individual oocytes, forming the very first cohort of primordial follicles. By Days 7 and 8, primary and early secondary follicles with intense TGF beta 2-positive cuboidal granulosa cells appeared. Subsequently, in large preantral follicles TGF beta 2 was expressed only in mural granulosa cells. On Day 13, TGF beta 1 and beta 2 immunoreactivities appeared for the first time in the interstitial cells. TGF beta 1 was localized in cells closely apposed to follicles, but TGF beta 2 activity was restricted to scattered cell clusters. Subsequently, the entire interstitium was positive for TGF beta 1 protein. These results suggest that differentiation of somatic cells into granulosa cells is the first event in ovarian morphogenesis; once the finite number of granulosa cells is selected, the residual cells differentiate into interstitium. Whether ovarian TGF beta 2 and TGF beta 1 are physiologically important in granulosa and interstitial cell differentiation needs further evaluation.
利用细胞类型特异性蛋白标志物，研究了卵巢体细胞向颗粒细胞、间质细胞和膜细胞谱系的分化，以及围产期可能与颗粒细胞分化相关的卵巢生长因子。收集产前（妊娠第12 - 15天）和产后（第1 - 30天）雌性仓鼠的卵巢，用于转化生长因子（TGF）β（TGFβ1、β2和β3）的免疫组织化学定位。通过放射免疫分析法检测促卵泡激素（FSH）、促黄体生成素（LH）、孕酮和雌二醇- 17β的血浆水平。产前仓鼠卵巢含有大量有丝分裂的卵母细胞和少量体细胞。只有一小部分体细胞表达几乎检测不到的TGFβ2活性。产后第1天血浆FSH水平可检测到，并逐渐升高，在第25天达到峰值，而LH直到第12天才升高，并在产后第20天达到平台期。产后第1天，TGFβ2免疫反应性仅定位于某些与原始卵母细胞紧密相邻的细胞中。产后第4天，扁平的、TGFβ2阳性细胞围绕单个卵母细胞，形成最早一批原始卵泡。到第7天和第8天，出现了具有强烈TGFβ2阳性立方颗粒细胞的初级和早期次级卵泡。随后，在大型窦前卵泡中，TGFβ2仅在壁颗粒细胞中表达。第13天，TGFβ1和β2免疫反应性首次出现在间质细胞中。TGFβ1定位于与卵泡紧密相邻的细胞中，但TGFβ2活性仅限于散在的细胞簇。随后，整个间质对TGFβ1蛋白呈阳性。这些结果表明，体细胞向颗粒细胞的分化是卵巢形态发生中的第一个事件；一旦选择了有限数量的颗粒细胞，剩余细胞就分化为间质。卵巢TGFβ2和TGFβ1在颗粒细胞和间质细胞分化中是否具有生理重要性，需要进一步评估。
