Sesso A, Azimovas S R, Ferreira M A
Department of Pathology, Faculty of Medicine, University of São Paulo, Brazil.
Biol Cell. 1994;81(2):165-76. doi: 10.1016/s0248-4900(94)80007-3.
Post-ER membranous structures are clearly observed in pancreases fixed with aldehydes and subsequently with reduced osmium. Close to the transitional rough ER, clusters of vesicles of approximately 56 nm diameter are consistently present. In some cells, tortuous tubules appear enmeshed by the approximately 56 nm vesicles and by irregular, vesicular formations. In freeze-fracture replicas, the membranes of the bulges and tubules that protrude from the transitional rough ER differ from those of the donor compartment. These protrusions are herein designated as the budding chamber of the transitional rough ER. Quantitative and qualitative observations performed previously and in the present study show that the P and E freeze-fracture faces of the outermost Golgi cisternal membrane possess patterns of texture that are unique among membranes. The P-face exhibits a very high density of intramembranous particles of dimensions among the smallest yet described; E-faces show rugosities and an unusually high density of intramembranous particles of normal size. The membranes of the budding chamber, the putative transport vesicles of approximately 56 nm diameter, the sinuous tubules and the vesicles of irregular size and shape exhibit P and E fracture faces with textures indistinguishable from those of the corresponding P and E faces of the outermost Golgi cisterna.
在用醛类固定并随后用还原锇固定的胰腺中，可以清楚地观察到内质网后膜结构。在靠近过渡性粗面内质网的地方，始终存在直径约56nm的囊泡簇。在一些细胞中，迂曲的小管似乎被直径约56nm的囊泡和不规则的泡状结构所包围。在冷冻蚀刻复制品中，从过渡性粗面内质网突出的凸起和小管的膜与供体区室的膜不同。这些突出物在此被称为过渡性粗面内质网的出芽腔。先前和本研究进行的定量和定性观察表明，最外层高尔基体扁平囊膜的P面和E面冷冻蚀刻面具有在膜中独特的纹理模式。P面显示出尺寸为迄今所描述的最小尺寸之一的膜内颗粒的非常高的密度；E面显示出皱纹和正常大小的膜内颗粒的异常高的密度。出芽腔的膜、直径约56nm的假定运输囊泡、蜿蜒的小管以及大小和形状不规则的囊泡的P面和E面冷冻蚀刻面显示出与最外层高尔基体扁平囊相应的P面和E面无法区分的纹理。
