Stafejeva O A, Katrukha A G, Bulargina T V, Khailova L S
A.N. Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow.
Biochem Mol Biol Int. 1994 Aug;34(1):9-15.
The monoclonal antibody F7F10 against the E1 component of the pigeon breast muscle pyruvate dehydrogenase complex has been produced. The dissociation constant of the E1-mAb F7F10 complex was determined to be 5.93 x 10(-8)M. The cross-reaction of the mAb with the E1 components of the pyruvate dehydrogenase complexes from various species (including human) was established. The competitive solid-phase immunoenzyme assay of the E1 component and PDC concentrations has been developed.
已制备出针对鸽胸肌丙酮酸脱氢酶复合体E1组分的单克隆抗体F7F10。测定E1 - mAb F7F10复合体的解离常数为5.93×10⁻⁸M。确定了该单克隆抗体与来自各种物种（包括人类）的丙酮酸脱氢酶复合体E1组分的交叉反应。已开发出E1组分和PDC浓度的竞争性固相免疫酶测定法。
