Thomas S R, Millard R W, Pratt R G, Shiferaw Y, Samaratunga R C
Department of Radiology, University of Cincinnati, Ohio 45267-0579.
Artif Cells Blood Substit Immobil Biotechnol. 1994;22(4):1029-42. doi: 10.3109/10731199409138800.
The physiological redistribution of perfluorocarbon (PFC) compounds to liver, spleen, bone marrow, and lung after intravenous (i.v.) or intraperitoneal (IP) administration of PFC emulsions affords the unique opportunity for non-invasive monitoring of oxygenation status of these organs and tissues utilizing fluorine (F-19) nuclear magnetic resonance (NMR) imaging techniques. PFCs also may be introduced directly into the pulmonary airways by procedures such as liquid ventilation, intratracheal instillation, or aerosol inhalation. Considerations of importance when establishing methodology for accurate quantitation of oxygen partial pressure (pO2) in vivo using F-19 NMR include: 1.) error analysis of the calibration curves which relate pO2 to the measured PFC F-19 relaxation rate, 2.) optimization of the NMR pulse sequence for efficient oxygen sensitive data acquisition and, 3.) fluorine signal independence from emulsion aqueous phase bioconstituents. The porcine model was investigated at 0.14T following i.v. or IP administration of the PFC emulsion containing perfluorotributylamine (FC-43) to demonstrate the capability for tracking oxygen with F-19 NMR from the lung through the blood to selected organ tissues. Quantitative pO2 projection images and isobaric contour graphs were derived for the liver, spleen, and lungs as a function of inspired oxygen. Blood pO2 levels in aorta, pulmonary artery, and hepatic vein were monitored simultaneously with NMR imaging for correlative analysis.
在静脉内（i.v.）或腹腔内（IP）给予全氟碳（PFC）乳剂后，PFC化合物会发生生理性重新分布至肝脏、脾脏、骨髓和肺，这为利用氟（F-19）核磁共振（NMR）成像技术对这些器官和组织的氧合状态进行非侵入性监测提供了独特机会。PFCs也可通过诸如液体通气、气管内滴注或气溶胶吸入等程序直接引入肺气道。在建立使用F-19 NMR体内准确定量氧分压（pO2）的方法时，重要的考虑因素包括：1.）将pO2与所测PFC F-19弛豫率相关联的校准曲线的误差分析；2.）优化NMR脉冲序列以高效采集对氧敏感的数据；3.）氟信号与乳剂水相生物成分无关。在0.14T磁场下，对猪模型静脉内或腹腔内给予含全氟三丁胺（FC-43）的PFC乳剂，以证明利用F-19 NMR从肺经血液追踪氧至选定器官组织的能力。得出了肝脏、脾脏和肺作为吸入氧函数的定量pO2投影图像和等压轮廓图。在进行NMR成像的同时监测主动脉、肺动脉和肝静脉中的血液pO2水平以进行相关分析。
