Tarara T E, Malinoff S H, Pelura T J
Alliance Pharmaceutical Corp., San Diego, CA 92121.
Artif Cells Blood Substit Immobil Biotechnol. 1994;22(4):1287-93. doi: 10.3109/10731199409138828.
Methods based on the HPLC separation with subsequent UV detection and spectrofluorimetry have been developed to monitor the formation of oxidative decomposition products of phospholipids in perfluorocarbon emulsions. Catalytic, as well as emulsion oxidative stability studies have been conducted utilizing egg yolk phospholipid (EYP) and perfluorocarbons of varied compositions/purity in order to assess their effect on susceptibility to oxidation. Our studies indicate that phospholipid composition, degree of unsaturation, perfluorocarbon purity and the presence of oxygen and trace metals have a significant effect on the formation of oxidative decomposition products. A combination of methods has proven useful in monitoring the levels of oxidative decomposition products of phospholipids in phospholipid-stabilized perfluorocarbon-based blood substitutes. Such an approach has proven beneficial in the development of pharmaceutical agents of potentially higher quality and storage stability.
基于高效液相色谱分离并随后进行紫外检测和荧光光谱法的方法已被开发出来，用于监测全氟碳乳液中磷脂氧化分解产物的形成。利用蛋黄磷脂（EYP）和不同组成/纯度的全氟碳进行了催化以及乳液氧化稳定性研究，以评估它们对氧化敏感性的影响。我们的研究表明，磷脂组成、不饱和度、全氟碳纯度以及氧气和痕量金属的存在对氧化分解产物的形成有显著影响。已证明多种方法相结合有助于监测磷脂稳定的全氟碳基血液替代品中磷脂氧化分解产物的水平。这种方法已被证明对开发质量和储存稳定性可能更高的药物制剂有益。
