Fernández-Tárrago J, Rodríguez-Buján M C, Nicolás G
Rev Esp Fisiol. 1978 Mar;34(1):87-92.
The variations in starch and soluble sugar content, in phosphorylase and amylase activities in cotyledons of germinating seeds of Cicer arietinum L. are determined. Results from various experiments prove that the alpha-amylases are chiefly responsible for amylase activity. Phosphorylase plays an important rôle during the first two days of germination, but it is relegated to a secondary position as the amylase activity increases. Disc electrophoresis on polyacrylamide gel shows the existence of a phosphorylase throughout germination, and detects two alpha-amylases after 48 and 96 h germination respectively. The increase in alpha-amylase activity during germination is due to de novo synthesis of the two isoenzymes, since both are inhibited by cycloheximide and actinomyces D. This de novo synthesis depends on some embryo produced factor, unreplaceable either by giberellic acid or by kinetin.
测定了鹰嘴豆萌发种子子叶中淀粉和可溶性糖含量以及磷酸化酶和淀粉酶活性的变化。各种实验结果证明，α-淀粉酶是淀粉酶活性的主要原因。磷酸化酶在萌发的前两天起重要作用，但随着淀粉酶活性的增加，它退居次要地位。聚丙烯酰胺凝胶圆盘电泳显示在整个萌发过程中存在一种磷酸化酶，分别在萌发48小时和96小时后检测到两种α-淀粉酶。萌发过程中α-淀粉酶活性的增加是由于两种同工酶的从头合成，因为两者都受到环己酰亚胺和放线菌素D的抑制。这种从头合成依赖于一些胚胎产生的因子，赤霉素或激动素都无法替代。
