Shinohara T, Taniwaki M, Ishida Y, Kawaichi M, Honjo T
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
Genomics. 1994 Oct;23(3):704-6. doi: 10.1006/geno.1994.1562.
A cDNA encoding mouse PD-1, a member of the immunoglobulin superfamily was previously isolated from apoptosis-induced cells by subtractive hybridization. To determine the structure and chromosomal location of the human PD-1 gene, we screened a human T cell cDNA library by mouse PD-1 probe and isolated a cDNA coding for the human PD-1 protein. The deduced amino acid sequence of human PD-1 was 60% identical to the mouse counterpart, and a putative tyrosine kinase-association motif was well conserved. The human PD-1 gene was mapped to 2q37.3 by chromosomal in situ hybridization.
编码小鼠程序性死亡蛋白1（PD-1）（免疫球蛋白超家族成员）的互补DNA（cDNA）先前已通过消减杂交技术从凋亡诱导细胞中分离出来。为了确定人类PD-1基因的结构和染色体定位，我们用小鼠PD-1探针筛选了人类T细胞cDNA文库，并分离出一个编码人类PD-1蛋白的cDNA。推导的人类PD-1氨基酸序列与小鼠对应序列有60%的同一性，并且一个假定的酪氨酸激酶结合基序保守性良好。通过染色体原位杂交将人类PD-1基因定位于2q37.3。
