Mount D W, Kosel C K, Walker A
Mol Gen Genet. 1976 Jul 5;146(1):37-41. doi: 10.1007/BF00267980.
Host of cell reactivation and UV reactivation and mutagenesis of UV-irradiated phage mu were measured in tsl recAplus and tsl recA host mutants. Host cell reactivation was slightly more efficient in the tsl recA strain. Phage was UV-reactivated in the tsl recA strain with about one-half the efficiency of that in the wild type strain, but there was no corresponding mutagenesis of phage. UV-reactivation was also slightly lower and mutagenesis several-fold lower than normal in the tsl recAplus strain. To account for these observations, we propose that there is an inducible, error-free pathway of DNA repair in E. coli that competes with error-prone repair for repair of phage lesions.
在tsl recA+和tsl recA宿主突变体中测定了细胞再激活、紫外线再激活以及紫外线照射的噬菌体μ的诱变情况。宿主细胞再激活在tsl recA菌株中效率略高。噬菌体在tsl recA菌株中被紫外线再激活，效率约为野生型菌株的一半，但噬菌体没有相应的诱变。在tsl recA+菌株中，紫外线再激活也略低于正常水平，诱变比正常情况低几倍。为了解释这些观察结果，我们提出大肠杆菌中存在一种可诱导的无差错DNA修复途径，它与易出错修复竞争以修复噬菌体损伤。
