在大肠杆菌中表达的玉米半胱氨酸蛋白酶抑制剂I：其抑制特性及在玉米粒中的存在情况研究
Corn cystatin I expressed in Escherichia coli: investigation of its inhibitory profile and occurrence in corn kernels.
作者信息
Abe M, Abe K, Iwabuchi K, Domoto C, Arai S
机构信息
Laboratory for Food Science, Gakushuin Women's Junior College, Tokyo.
出版信息
J Biochem. 1994 Sep;116(3):488-92. doi: 10.1093/oxfordjournals.jbchem.a124551.
Corn cystatin I was expressed in Escherichia coli as a mature protein. It was purified by gel filtration on Sephadex G-50, ion-exchange HPLC, and reversed-phase HPLC. The purified protein showed strong inhibitory activities against papain (Ki: 3.7 x 10(-8) M), and cathepsins H (Ki: 5.7 x 10(-9) M) and L (Ki: 1.7 x 10(-8) M), whereas it inhibited cathepsin B to a lesser extent (Ki: 2.9 x 10(-7) M). Western blot analysis using antibody raised against corn cystatin I revealed that in the corn kernel, the protein occurs with a molecular mass of approximately 13 kDa. Localization in the aleurone layer and embryo of the corn kernel was shown by immunostaining microscopy.
玉米半胱氨酸蛋白酶抑制剂I作为成熟蛋白在大肠杆菌中表达。通过在Sephadex G - 50上进行凝胶过滤、离子交换高效液相色谱和反相高效液相色谱进行纯化。纯化后的蛋白对木瓜蛋白酶（Ki：3.7×10⁻⁸ M）、组织蛋白酶H（Ki：5.7×10⁻⁹ M）和L（Ki：1.7×10⁻⁸ M）表现出强烈的抑制活性，而对组织蛋白酶B的抑制程度较小（Ki：2.9×10⁻⁷ M）。使用针对玉米半胱氨酸蛋白酶抑制剂I产生的抗体进行的蛋白质印迹分析表明，在玉米粒中，该蛋白的分子量约为13 kDa。免疫染色显微镜显示了其在玉米粒糊粉层和胚中的定位。
Zotero 插件