Ishikoh A U, Hayashi A, Tokimitsu I, Tajima S, Nishikawa T
Department of Dermatology, Keio University School of Medicine, Toky.
J Biochem. 1994 Sep;116(3):610-4. doi: 10.1093/oxfordjournals.jbchem.a124568.
Two clones, melanotic (M3) and amelanotic (A4) cells, were isolated from B16 mouse melanoma cells. The two clones exhibited distinct phenotypes in cell morphology, melanogenesis, and secretion of extracellular matrix, with preferential secretion of type I trimer collagen in A4 cells. Grown on type I collagen substratum, M3 cells were converted to cells exhibiting similar phenotypes to A4 cells. Subsequent culture of A4-like cells on plastic dishes resulted in the recovery of the initial M3 phenotypes. These results indicate that the reversible conversion between melanotic and amelanotic cells in B16 melanoma cells in vitro is controlled by type I collagen substratum and that modulation of cell shape, melanogenesis, and type I trimer collagen secretion during conversion were all coordinately and reversibly regulated. The results provide evidence that extracellular matrix can control the cell phenotypes in mouse B16 melanoma. Modulation of type I trimer collagen secretion by type I collagen substrate could be a useful model for studying the alpha 1(I) chain-specific regulatory mechanism.
从B16小鼠黑色素瘤细胞中分离出两个克隆，即黑色素瘤细胞（M3）和无黑色素瘤细胞（A4）。这两个克隆在细胞形态、黑色素生成和细胞外基质分泌方面表现出不同的表型，A4细胞优先分泌I型三聚体胶原蛋白。在I型胶原蛋白基质上生长时，M3细胞转变为表现出与A4细胞相似表型的细胞。随后将类似A4的细胞培养在塑料培养皿上，又恢复了最初的M3表型。这些结果表明，体外培养的B16黑色素瘤细胞中黑色素瘤细胞和无黑色素瘤细胞之间的可逆转化受I型胶原蛋白基质控制，并且转化过程中细胞形态、黑色素生成和I型三聚体胶原蛋白分泌的调节都是协调且可逆的。这些结果提供了证据，证明细胞外基质可以控制小鼠B16黑色素瘤中的细胞表型。I型胶原蛋白底物对I型三聚体胶原蛋白分泌的调节可能是研究α1（I）链特异性调节机制的有用模型。
