Akiu S, Suzuki Y, Asahara T, Fujinuma Y, Fukuda M
Basic Research Laboratories, Shiseido Research Center.
Nihon Hifuka Gakkai Zasshi. 1991 May;101(6):609-13.
Inhibitory effect of arbutin (hydroquinone-beta-D-glucopyranoside) on the melanogenesis was studied biochemically using cultured B16 melanoma cells. The maximum arbutin concentration lacking an inhibitory effect on cell growth was 5 X 10(-5) M. At this concentration, melanin content per cell was decreased significantly to about 39%, compared with that of arbutin untreated cells. Also, tyrosinase activity of arbutin treated cells was decreased significantly. When arbutin was added to B16 melanoma cell suspension, arbutin was not hydrolyzed to liberate hydroquinone. Further, tyrosinase activity in crude preparations from B16 melanoma cells was inhibited by arbutin. From these results, it is suggested that arbutin can inhibit the melanogenesis by affecting not only the synthesis but also the activity of tyrosinase rather than by killing melanocytes B16 melanoma cells. Also, it is suggested that hydroquinone is not responsible for the inhibitory effect of arbutin on the melanogenesis.
利用培养的B16黑色素瘤细胞，对熊果苷（对苯二酚 - β - D - 吡喃葡萄糖苷）对黑色素生成的抑制作用进行了生化研究。对细胞生长无抑制作用的熊果苷最大浓度为5×10⁻⁵ M。在此浓度下，与未用熊果苷处理的细胞相比，每个细胞的黑色素含量显著降低至约39%。此外，用熊果苷处理的细胞的酪氨酸酶活性也显著降低。当将熊果苷添加到B16黑色素瘤细胞悬液中时，熊果苷不会水解以释放对苯二酚。此外，B16黑色素瘤细胞粗制品中的酪氨酸酶活性受到熊果苷的抑制。从这些结果表明，熊果苷不仅可以通过影响酪氨酸酶的合成，还可以通过影响其活性来抑制黑色素生成，而不是通过杀死B16黑色素瘤细胞中的黑素细胞。此外，表明对苯二酚与熊果苷对黑色素生成的抑制作用无关。
