Morse L S, Beck L A, Pauling C
Mol Gen Genet. 1976 Aug 10;147(1):79-82. doi: 10.1007/BF00337939.
We have examined DNA strand breakage, DNA degradation, and the rate of DNA synthesis in lig and lig-recB strains of Escherichia coli K12 incubated in the presence and absence of 3 mug/ml chloramphenicol. Substantial DNA strand breakage and DNA degradation is observed in the lig strain upon growth at 40 degrees C; however, such strand breakage and DNA degradation is not observed in th lig-recB strainl Incubation of the lig strain at 40 degrees C in the presence of 3 mug/ml chloramphenicol reduces the amount of DNA strand breakage and DNA degradation to the level observed in the lig-recB strain. Together, these results demonstrate that exonuclease V (the recBC gene product) is responsible for the increased DNA degradation associated with DNA ligase deficiency.
我们检测了在有和没有3微克/毫升氯霉素存在的情况下培养的大肠杆菌K12的连接酶（lig）和连接酶-重组酶B（lig-recB）菌株中的DNA链断裂、DNA降解以及DNA合成速率。在40℃生长时，在连接酶菌株中观察到大量的DNA链断裂和DNA降解；然而，在连接酶-重组酶B菌株中未观察到这种链断裂和DNA降解。在3微克/毫升氯霉素存在的情况下，将连接酶菌株在40℃培养，可将DNA链断裂和DNA降解的量降低至连接酶-重组酶B菌株中观察到的水平。这些结果共同表明，外切核酸酶V（recBC基因产物）是导致与DNA连接酶缺陷相关的DNA降解增加的原因。
