噬菌体Mu恢复大肠杆菌K12 lig ts7菌株中的连接酶活性并克隆携带Mu“lig”基因的DNA片段。
Restoration of ligase activity in E. coli K12 lig ts7 strain by bacteriophage Mu and cloning of a DNA fragment harbouring the Mu 'lig' gene.
作者信息
Ghelardini P, Paolozzi L, Liebart J C
出版信息
Nucleic Acids Res. 1980 Jul 25;8(14):3157-73. doi: 10.1093/nar/8.14.3157.
Abstract
Restoration of ligase activity has been observed in E. coli K12 ligts7 strain lysogenic for Mu, in presence as well in absence of lysogenic immunity. This restoration consist in phenotypic reversal of temperature sensitivity of E. coli ligts7 which also regain the ability to sustain the complete growth cycle of T4 lig-phages. It is possible to put under the control of the gal operon the expression of the viral gene responsible for the restoration effect. This new gene of Mu has been named 'lig'. A 5 kb fragment responsible for the reported effects and localized between genes gam and lys of Mu genome has been cloned in pBR322. This recombinant plasmid used for transforming ligts7 strain restores in it normal behaviour for ligation of Okazaki pieces.
摘要
在对Mu具有溶原性的大肠杆菌K12 ligts7菌株中，无论有无溶原免疫性，都观察到了连接酶活性的恢复。这种恢复表现为大肠杆菌ligts7温度敏感性的表型逆转，它也重新获得了维持T4连接噬菌体完整生长周期的能力。负责恢复效应的病毒基因的表达可以置于半乳糖操纵子的控制之下。Mu的这个新基因被命名为“lig”。一个负责上述效应且位于Mu基因组gam和lys基因之间的5kb片段已被克隆到pBR322中。用于转化ligts7菌株的这种重组质粒恢复了其连接冈崎片段的正常行为。
相似文献
1
Restoration of ligase activity in E. coli K12 lig ts7 strain by bacteriophage Mu and cloning of a DNA fragment harbouring the Mu 'lig' gene.噬菌体Mu恢复大肠杆菌K12 lig ts7菌株中的连接酶活性并克隆携带Mu“lig”基因的DNA片段。
Nucleic Acids Res. 1980 Jul 25;8(14):3157-73. doi: 10.1093/nar/8.14.3157.
2
Suppression of the thermosensitive DNA ligase mutations in Escherichia coli K12 through modulation of gene expression induced by phage Mu.通过调节噬菌体Mu诱导的基因表达抑制大肠杆菌K12中的热敏DNA连接酶突变。
Mol Gen Genet. 1989 Mar;216(1):31-6. doi: 10.1007/BF00332227.
3
[Ligase activity of lig- bacteria infected with mu bacteriophage at non-permissive temperatures].[在非允许温度下感染了μ噬菌体的连接酶缺陷细菌的连接酶活性]
C R Seances Acad Sci D. 1979 Jan 15;288(2):259-62.
4
Two classes of Mu lig mutants: the thermosensitives for integration and replication and the hyperproducers for ligase.两类穆氏连接酶（Mu lig）突变体：整合与复制的温度敏感型以及连接酶的高产型。
Nucleic Acids Res. 1980 Dec 11;8(23):5859-73. doi: 10.1093/nar/8.23.5859.
5
Restoration of DNA synthesis at non-permissive temperature and of UV resistance induced by bacteriophage Mu in Escherichia coli lig ts7 strain.在大肠杆菌连接酶ts7菌株中，噬菌体Mu诱导的非允许温度下DNA合成的恢复以及紫外线抗性的恢复。
Ann Microbiol (Paris). 1979 Oct;130B(3):275-85.
6
DNA ligase is required for encapsidation of bacteriophage T4 DNA.噬菌体T4 DNA的衣壳化需要DNA连接酶。
J Mol Biol. 1981 Jul 15;149(4):641-58. doi: 10.1016/0022-2836(81)90351-x.
7
Sealing of gaps in duplex DNA by T4 DNA ligase.利用T4 DNA连接酶封闭双链DNA中的缺口。
Nucleic Acids Res. 1982 Mar 11;10(5):1425-37. doi: 10.1093/nar/10.5.1425.
8
Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product.噬菌体T4 DNA连接酶基因的分子克隆。II. 基因产物的扩增与制备。
J Mol Biol. 1979 Aug 15;132(3):493-505. doi: 10.1016/0022-2836(79)90271-7.
9
Expression of the bacteriophage T4 denV structural gene in Escherichia coli.噬菌体T4 denV结构基因在大肠杆菌中的表达。
J Bacteriol. 1986 Nov;168(2):1014-8. doi: 10.1128/jb.168.2.1014-1018.1986.
10
Genome mapping and protein coding region identification using bacteriophage Mu.利用噬菌体Mu进行基因组图谱绘制和蛋白质编码区鉴定。
Gene. 1991 Mar 1;99(1):1-7. doi: 10.1016/0378-1119(91)90026-8.
引用本文的文献
1
Crystallization and preliminary X-ray analysis of gene product 44 from bacteriophage Mu.噬菌体Mu基因产物44的结晶及初步X射线分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt 1):104-5. doi: 10.1107/S1744309104029574. Epub 2004 Dec 24.
2
The Mu gem operon: its role in gene expression, recombination and cell cycle.Mu 基因操纵子：其在基因表达、重组及细胞周期中的作用
Genetica. 1994;94(2-3):151-6. doi: 10.1007/BF01443429.
3
Bacteriophage Mu DNA replication is stimulated by non-essential early functions.噬菌体Mu DNA复制受非必需早期功能的刺激。
Mol Gen Genet. 1982;186(1):135-9. doi: 10.1007/BF00422925.
4
Two classes of Mu lig mutants: the thermosensitives for integration and replication and the hyperproducers for ligase.两类穆氏连接酶（Mu lig）突变体：整合与复制的温度敏感型以及连接酶的高产型。
Nucleic Acids Res. 1980 Dec 11;8(23):5859-73. doi: 10.1093/nar/8.23.5859.
5
Escherichia coli K-12 gyrB gene product is involved in the lethal effect of the ligts2 mutant of bacteriophage Mu.大肠杆菌K-12 gyrB基因产物参与噬菌体Mu的ligts2突变体的致死效应。
J Bacteriol. 1984 Feb;157(2):665-8. doi: 10.1128/jb.157.2.665-668.1984.
6
Purification of the gam gene-product of bacteriophage Mu and determination of the nucleotide sequence of the gam gene.噬菌体Mu的gam基因产物的纯化及gam基因核苷酸序列的测定。
Nucleic Acids Res. 1986 Sep 11;14(17):6901-14. doi: 10.1093/nar/14.17.6901.
7
Suppression of the thermosensitive DNA ligase mutations in Escherichia coli K12 through modulation of gene expression induced by phage Mu.通过调节噬菌体Mu诱导的基因表达抑制大肠杆菌K12中的热敏DNA连接酶突变。
Mol Gen Genet. 1989 Mar;216(1):31-6. doi: 10.1007/BF00332227.
8
Synchronous division induced in Escherichia coli K12 by gemts mutants of phage Mu.噬菌体Mu的gemts突变体在大肠杆菌K12中诱导的同步分裂。
Mol Gen Genet. 1989 Jul;218(1):13-7. doi: 10.1007/BF00330559.
本文引用的文献
1
Segregation of Lambda Lysogenicity during Bacterial Recombination in Escherichia Coli K12.大肠杆菌K12中细菌重组过程中λ原噬菌体溶源性的分离
Genetics. 1954 Jul;39(4):429-39. doi: 10.1093/genetics/39.4.429.
2
Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles.大肠杆菌和痢疾志贺氏菌菌株间乳糖利用能力的转导及转导噬菌体颗粒的特性
Virology. 1960 Nov;12:348-90. doi: 10.1016/0042-6822(60)90161-6.
3
Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase.具有改变的DNA连接酶的大肠杆菌突变体的酶学特性分析。
Proc Natl Acad Sci U S A. 1971 May;68(5):1002-5. doi: 10.1073/pnas.68.5.1002.
4
Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.DNA链生长的机制。I. 新合成链可能的不连续性和异常二级结构。
Proc Natl Acad Sci U S A. 1968 Feb;59(2):598-605. doi: 10.1073/pnas.59.2.598.
5
The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis.枯草芽孢杆菌的染色体。I. 标记频率分析理论
Genetics. 1965 Oct;52(4):747-57. doi: 10.1093/genetics/52.4.747.
6
Properties of an R factor from Pseudomonas aeruginosa.铜绿假单胞菌R因子的特性
J Bacteriol. 1971 Dec;108(3):1244-9. doi: 10.1128/jb.108.3.1244-1249.1971.
7
Electron microscopic evidence for linear insertion of bacteriophage MU-1 in lysogenic bacteria.噬菌体MU-1在溶原性细菌中线性插入的电子显微镜证据。
J Virol. 1971 Oct;8(4):551-63. doi: 10.1128/JVI.8.4.551-563.1971.
8
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.聚乙二醇存在下噬菌体的快速沉降及其在大规模病毒纯化中的应用
Virology. 1970 Mar;40(3):734-44. doi: 10.1016/0042-6822(70)90218-7.
9
10
Properties of a temperature-sensitive radiation-sensitive mutant of Escherichia coli.大肠杆菌温度敏感辐射敏感突变体的特性
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1495-502. doi: 10.1073/pnas.60.4.1495.
Zotero 插件