噬菌体Mu恢复大肠杆菌K12 lig ts7菌株中的连接酶活性并克隆携带Mu“lig”基因的DNA片段。
Restoration of ligase activity in E. coli K12 lig ts7 strain by bacteriophage Mu and cloning of a DNA fragment harbouring the Mu 'lig' gene.
作者信息
Ghelardini P, Paolozzi L, Liebart J C
出版信息
Nucleic Acids Res. 1980 Jul 25;8(14):3157-73. doi: 10.1093/nar/8.14.3157.
Abstract
Restoration of ligase activity has been observed in E. coli K12 ligts7 strain lysogenic for Mu, in presence as well in absence of lysogenic immunity. This restoration consist in phenotypic reversal of temperature sensitivity of E. coli ligts7 which also regain the ability to sustain the complete growth cycle of T4 lig-phages. It is possible to put under the control of the gal operon the expression of the viral gene responsible for the restoration effect. This new gene of Mu has been named 'lig'. A 5 kb fragment responsible for the reported effects and localized between genes gam and lys of Mu genome has been cloned in pBR322. This recombinant plasmid used for transforming ligts7 strain restores in it normal behaviour for ligation of Okazaki pieces.
摘要
在对Mu具有溶原性的大肠杆菌K12 ligts7菌株中，无论有无溶原免疫性，都观察到了连接酶活性的恢复。这种恢复表现为大肠杆菌ligts7温度敏感性的表型逆转，它也重新获得了维持T4连接噬菌体完整生长周期的能力。负责恢复效应的病毒基因的表达可以置于半乳糖操纵子的控制之下。Mu的这个新基因被命名为“lig”。一个负责上述效应且位于Mu基因组gam和lys基因之间的5kb片段已被克隆到pBR322中。用于转化ligts7菌株的这种重组质粒恢复了其连接冈崎片段的正常行为。
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