Jones D S, Lundgren H K, Jay F T
Nucleic Acids Res. 1976 Jun;3(6):1569-76. doi: 10.1093/nar/3.6.1569.
Lysine-agarose provides a simple means of separating RNA species of different moleecular weight. When RNA from Escherichia coli is added to a small column of lysine-agarose and elution is carried out at neutral pH with a shallow linear gradient of NaCl the RNA species are eluted according to size; 4S and 5S RNA species are completely separated and after a delay the 16S and 23S species are eluted as separate peaks. The process is very reproducible and the different species are eluted at a fixed salt concentration even when changes are made in the gradient, provided other conditions, under which the column is run remain constant.
赖氨酸琼脂糖提供了一种分离不同分子量RNA种类的简单方法。当将来自大肠杆菌的RNA添加到一小柱赖氨酸琼脂糖中，并在中性pH下用NaCl的浅线性梯度进行洗脱时，RNA种类会根据大小被洗脱；4S和5S RNA种类被完全分离，经过一段时间延迟后，16S和23S种类作为单独的峰被洗脱。该过程具有很高的可重复性，即使在梯度发生变化时，不同种类的RNA也会在固定的盐浓度下被洗脱，前提是柱运行的其他条件保持不变。
