Schmidt-Kastner R, Tomac A, Hoffer B, Bektesh S, Rosenzweig B, Olson L
Department of Neuroscience, Histology, Karolinska Institute, Stockholm, Sweden.
Brain Res Mol Brain Res. 1994 Oct;26(1-2):325-30. doi: 10.1016/0169-328x(94)90106-6.
Glial cell-line derived neurotrophic factor (GDNF) has recently been cloned and shown to have trophic effects on dopaminergic nigral neurons. However, GDNF mRNA has not been detected in striatum or other forebrain areas of adult rat. Using limbic motor status epilepticus induced by pilocarpine to activate neurons in motor and limbic areas, we now demonstrate GDNF mRNA signals in the striatum, hippocampus and cortex using in situ hybridisation. The finding of GDNF mRNA in the stimulated striatum opens the possibility that GDNF may be a target-derived, trophic factor in the nigro-striatal system. This expression of GDNF mRNA may be linked to excitatory cortical input. Increases in GDNF mRNA after status epilepticus in hippocampus and neocortex indicate additional roles for GDNF.
胶质细胞源性神经营养因子（GDNF）最近已被克隆，并显示对黑质多巴胺能神经元具有营养作用。然而，在成年大鼠的纹状体或其他前脑区域未检测到GDNF mRNA。利用毛果芸香碱诱导的边缘性运动性癫痫持续状态激活运动和边缘区域的神经元，我们现在通过原位杂交在纹状体、海马体和皮质中证实了GDNF mRNA信号。在受刺激的纹状体中发现GDNF mRNA，提示GDNF可能是黑质 - 纹状体系统中一种靶源性营养因子。GDNF mRNA的这种表达可能与兴奋性皮质输入有关。癫痫持续状态后海马体和新皮质中GDNF mRNA的增加表明GDNF还有其他作用。
