Ikegaki N, Saito N, Hashima M, Tanaka C
Department of Pharmacology, Kobe University School of Medicine, Japan.
Brain Res Mol Brain Res. 1994 Oct;26(1-2):47-54. doi: 10.1016/0169-328x(94)90072-8.
Polyclonal subtype-specific antibodies were developed against three subtypes of GABA transporters (GAT1, GAT2 and GAT3). By immunoblot analysis, each antibody detected a single band that could be blocked by absorption of the antibody with the respective antigen. GAT2 was found in various tissues, while GAT1 and GAT3 were detected only in the brain. GAT1 was distributed throughout the brain with the highest amount in the olfactory bulb, CA3 region of the hippocampus, layer I of the cerebral cortex, piriform cortex, superior colliculus, interpeduncular nucleus and nucleus spinal tract of the trigeminal nerve, while the GAT3 was densely found in the olfactory bulb, thalamus, hypothalamus, pons and medulla, globus pallidus, central gray, substantia nigra, deep cerebellar nuclei and nucleus spinal tract of the trigeminal nerve but not in the hippocampus, cerebral cortex, caudate-putamen and cerebellar cortex. GAT2 immunoreactivity was faint throughout the brain but was concentrated in the arachnoid and ependymal cells. Both GAT1 and GAT3 were found in the neuropil but not in the cell bodies nor in the white matter. These results suggest that GAT1, GAT2 and GAT3 are expressed in different cells and that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be related to non-neuronal function.
针对三种γ-氨基丁酸转运体亚型（GAT1、GAT2和GAT3）研制了多克隆亚型特异性抗体。通过免疫印迹分析，每种抗体均检测到一条单一的条带，该条带可被相应抗原吸收抗体所阻断。GAT2存在于多种组织中，而GAT1和GAT3仅在脑中检测到。GAT1分布于整个脑，在嗅球、海马体CA3区、大脑皮层I层、梨状皮层、上丘、脚间核和三叉神经脊髓束核中含量最高，而GAT3在嗅球、丘脑、下丘脑、脑桥和延髓、苍白球、中央灰质、黑质、小脑深部核团和三叉神经脊髓束核中密集存在，但在海马体、大脑皮层、尾状核-壳核和小脑皮层中不存在。GAT2免疫反应性在整个脑中较弱，但集中在蛛网膜和室管膜细胞中。GAT1和GAT3均存在于神经毡中，但不存在于细胞体和白质中。这些结果表明，GAT1、GAT2和GAT3在不同细胞中表达，GAT1和GAT3参与不同的γ-氨基丁酸能传递，而GAT2可能与非神经元功能有关。
