Engen P C, Wheeler R
Stain Technol. 1978 Jan;53(1):17-22. doi: 10.3109/10520297809111438.
A method of tissue embedding using n-butyl methacrylate and paraffin is described. Following alcohol dehydration and infiltration with the methacrylate monomer, tissues are embedded in gelatin capsules in a mixture consisting of 3.5 g of paraffin for each 10 ml of methacrylate. Benzoyl peroxide (0.2 g for each 10 ml of monomer) is added as the catalyst and the methacrylate polymerized in a 50 C oven for 18--24 h. Following polymerization the block is trimmed and embedded in paraffin to provide a firm support during sectioning. A water trough attached to the microtome knife is essential to facilitate the handling of sections and ribbons. For serial sections a mixture of equal weights of beeswax and paraffin is used to make the sections adhere to each other. Usual staining procedures can be used since the embedding medium is readily soluble in xylene.
描述了一种使用甲基丙烯酸正丁酯和石蜡进行组织包埋的方法。经过酒精脱水并用甲基丙烯酸酯单体浸润后，将组织包埋于明胶胶囊中，该混合物由每10毫升甲基丙烯酸酯对应3.5克石蜡组成。加入过氧化苯甲酰（每10毫升单体加0.2克）作为催化剂，甲基丙烯酸酯在50℃烘箱中聚合18 - 24小时。聚合后，修整组织块并将其包埋于石蜡中，以便在切片过程中提供坚实的支撑。连接到切片刀的水槽对于便于处理切片和条带至关重要。对于连续切片，使用等重量的蜂蜡和石蜡的混合物以使切片相互黏附。由于包埋介质易溶于二甲苯，因此可以使用常规染色程序。
