Bernhardt H, Zimmermann K, Knoke M
Klinik und Poliklinik für Innere Medizin, Ernst-Moritz-Arndt-Universität Greifswald.
Mycoses. 1994;37 Suppl 1:50-6.
We investigated the opportunistic pathogen Candida albicans in a continuous flow culture with regard to particular growth conditions. Germ counts of only 10(7) cfu/ml were obtained in a steady state of an anaerobic culture. In a parallel experiment with aerobic conditions excessive growth occurred and the invasive forms of Candida, i.e. germ tubes and pseudomycelium, also developed. Neither by different carbohydrates and concentration of nutrients in the culture medium nor by changing the flow rate the growth of Candida could be influenced. Excessive overgrowth was triggered by a sufficient oxygen supply.
我们在连续流动培养中研究了机会性病原体白色念珠菌的特定生长条件。在厌氧培养的稳定状态下,仅获得了10⁷ cfu/ml的菌数。在有氧条件的平行实验中,出现了过度生长,并且念珠菌的侵袭形式,即芽管和假菌丝也得以发育。无论是通过培养基中不同的碳水化合物和营养成分浓度,还是通过改变流速,都无法影响念珠菌的生长。充足的氧气供应引发了过度生长。