Ribaud C, Garson J C, Doucet J, Lévêque J L
Laboratories de Recherche de L'Oréal, Aulnay-Sous-Bois, France.
Pharm Res. 1994 Oct;11(10):1414-8. doi: 10.1023/a:1018987721531.
The role of the structural organization of intercorneocyte lipids in the barrier function of human stratum corneum was evaluated by treatment with heat and sodium lauryl sulfate. Measurement of transepidermal water loss in treated samples was used to quantify variations in stratum corneum permeability. Thermodynamic transition of lamellar lipids and their degree of organization were evaluated by differential scanning calorimetry and small-angle X-ray diffraction, respectively. Progressively preheating stratum corneum samples from 75 degrees C to 90 degrees C increased stratum corneum permeability to water vapor, while the fusion temperature of lamellar lipids and the intensity of the X-ray diffraction peaks of the polar lipids decreased. Sodium lauryl sulfate induced similar variations of these three parameters. These results support the hypothesis that, in addition to the chemical nature of intercorneocyte lipids, their structural arrangement and thermodynamic properties play an important role in the barrier function of the stratum corneum to water vapor.
通过加热和十二烷基硫酸钠处理，评估了角质形成细胞间脂质的结构组织在人类角质层屏障功能中的作用。通过测量处理后样品的经表皮水分流失来量化角质层通透性的变化。分别使用差示扫描量热法和小角X射线衍射评估层状脂质的热力学转变及其组织程度。将角质层样品从75摄氏度逐步预热至90摄氏度会增加角质层对水蒸气的通透性，而层状脂质的融合温度和极性脂质的X射线衍射峰强度会降低。十二烷基硫酸钠会引起这三个参数的类似变化。这些结果支持了以下假设：除了角质形成细胞间脂质的化学性质外，它们的结构排列和热力学性质在角质层对水蒸气的屏障功能中也起着重要作用。
