Davies H S, Taylor J E, White D J, Binns R M
Transplantation. 1978 Jun;25(6):290-5. doi: 10.1097/00007890-197806000-00002.
We have previously shown that cultured renal and hepatic parenchymal cells of the pig differ in the expression of major transplantation antigens. Antigens apparently analogous to those of the mouse I region were detectable on kidney and some peripheral blood lymphocytes but not on liver, platelets, or other peripheral blood lymphocytes. The present study confirms these findings for liver parenchymal cells and for a mixture of kidney tubules and glomeruli prepared from fresh, perfused whole organs. However, homogenates prepared at the same time from the perfused whole organs showed no antigenic disparity. Whole liver homogenate contains I region analogues that are barely detectable on the parenchymal cell membrane. Liver parencymal cells may possess such antigens in a form not patnetly expressed, or the antigens may belong to other types of cell resident in the liver which are not removed by extensive perfusion. Similar results were obtained with liver homogenates derived from an orthotopic transplant to a fully allogeneic recipient and resident for 37 days, suggesting that blood lymphocytes persisting in the normal liver after perfusion could not account for these findings.
我们之前已经表明，猪的培养肾实质细胞和肝实质细胞在主要移植抗原的表达上存在差异。在肾脏和一些外周血淋巴细胞上可检测到明显类似于小鼠I区的抗原，但在肝脏、血小板或其他外周血淋巴细胞上则检测不到。本研究证实了肝实质细胞以及从新鲜灌注的全器官制备的肾小管和肾小球混合物的这些发现。然而，同时从灌注的全器官制备的匀浆未显示出抗原差异。全肝匀浆含有在实质细胞膜上几乎检测不到的I区类似物。肝实质细胞可能以未明显表达的形式拥有此类抗原，或者这些抗原可能属于肝脏中其他类型的驻留细胞，而广泛灌注并不能将其去除。对原位移植到完全异基因受体并存活37天的肝脏匀浆也得到了类似结果，这表明灌注后正常肝脏中持续存在的血淋巴细胞无法解释这些发现。
