Duangchanda S, Tanaka M, Morita K, Rojanasuphot S, Igarashi A
Department of Virology, Nagasaki University, Japan.
Southeast Asian J Trop Med Public Health. 1994 Jun;25(2):243-51.
The nucleotide (nt) sequence of the envelope glycoprotein (E) gene of dengue virus type 2 was determined by the primer-extension dideoxy chain-termination method for 3 dengue virus type 2 (D2) strains which had been isolated from patients with dengue fever (DF), dengue hemorrhagic fever (DHF), and dengue shock syndrome (DSS), in Maha Sarakham, Northeast Thailand, in 1986-1987. Their nt sequences were essentially the same except for a single silent nt replacement in each DHF and DSS strain compared with DF strain. Therefore, these 3 strains possessed identical deduced amino acid (AA) sequences in their E protein. The result indicated that the primary structure of the E protein of D2 virus is not related to the clinical severity of the infected patients. Eleven nt replacements which resulted in 4 amino acid replacements were found to be unique to these 3 Northeast Thai strains. Sequence similarity showed that the 3 Northeast Thai strains were closest to the DSS isolate (H) followed by the DHF isolate (D) identified in Bangkok in 1980.
采用引物延伸双脱氧链终止法，对1986 - 1987年在泰国东北部玛哈沙拉堪从登革热（DF）、登革出血热（DHF）和登革休克综合征（DSS）患者中分离出的3株2型登革病毒的包膜糖蛋白（E）基因的核苷酸（nt）序列进行了测定。与DF株相比，除了在每株DHF和DSS株中有一个沉默的nt替换外，它们的nt序列基本相同。因此，这3株病毒在其E蛋白中具有相同的推导氨基酸（AA）序列。结果表明，D2病毒E蛋白的一级结构与感染患者的临床严重程度无关。发现这3株泰国东北部毒株特有的11个nt替换导致了4个氨基酸替换。序列相似性表明，这3株泰国东北部毒株与1980年在曼谷鉴定的DSS分离株（H）最接近，其次是DHF分离株（D）。
