[广东省三株登革病毒1型分离株E基因序列分析]
[Analysis of the E gene sequences of three dengue virus type 1 isolates in Guangdong province].
作者信息
Tian Xiao-dong, Liu Jian-wei, Fang Mei-yu, Jiang Lian-hua, Ren Rui-wen, Hong Wen-yan, Cheng Gang-feng
机构信息
Department of Microbiology, Institute of Military Medicine of Guangzhou Command, Guangzhou 510507, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2005 Jan;25(1):54-7.
OBJECTIVE
To trace the infection sources of three dengue virus type 1 isolates found in Guangdong province, namely GD05/99, GD14/97 and GD23/95.
METHODS
According to the genomes of dengue virus type 1 S275 strain, a pair of primers was designed for amplification of the structural protein E gene of the isolated dengue virus type 1 strains by reverse transcriptase-PCR. The amplified gene fragment was then cloned into pMD18-T vector and sequenced.
RESULTS
GD14/97 and GD05/99 shared a high nucleotide homology with Cambodia (98% and 99% respectively) and may belong to the same genotype. GD23/95 had a 95% nucleotide homology with A88, higher than that with other strains. GD23\95 and A88 may belong to another genotype.
CONCLUSION
The dengue fever outbreak in Guangdong during 1999, 1997, and 1995 may originate from the viruses from different countries.
目的
追踪在广东省发现的三株1型登革病毒分离株，即GD05/99、GD14/97和GD23/95的感染源。
方法
根据1型登革病毒S275株的基因组，设计一对引物，通过逆转录-聚合酶链反应（RT-PCR）扩增分离的1型登革病毒株的结构蛋白E基因。然后将扩增的基因片段克隆到pMD18-T载体中并测序。
结果
GD14/97和GD05/99与柬埔寨的核苷酸同源性较高（分别为98%和99%），可能属于同一基因型。GD23/95与A88的核苷酸同源性为95%，高于与其他毒株的同源性。GD23/95和A88可能属于另一个基因型。
结论
1999年、1997年和1995年广东省的登革热疫情可能源自不同国家的病毒。
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