Thome M, Hirt W, Pfaff E, Reddehase M J, Saalmüller A
Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
Vet Immunol Immunopathol. 1994 Oct;43(1-3):13-8. doi: 10.1016/0165-2427(94)90115-5.
Two subclasses of CD3 associated T-cell receptors (TcR) have been described so far, consisting of either an alpha and beta chain (TcR alpha beta) or a gamma and delta chain (TcR gamma delta). Of the two subclasses, the TcR alpha beta is the one predominantly expressed on peripheral T lymphocytes of humans and rodents. TcR gamma delta T lymphocytes represent only a minor subset in these species. Among all mammalian species studied so far, swine showed the most diversified composition of the T-lymphocyte population characterized by the expression of CD4 and CD8 differentiation antigens. Besides CD4+CD8- and CD4-CD8+ T lymphocytes, CD4+CD8+ and CD4-CD8- T lymphocytes are prominent in the extrathymic T-lymphocyte compartment. Because of the lack of specific monoclonal antibodies (mAb), to date the porcine TcR can only be characterized with biochemical and molecular biological methods. TcR on porcine peripheral blood T lymphocytes with the phenotype CD4+ and/or CD8+ are characterized as 46-48 kDaR heterodimers which were supposed to represent the porcine TcR alpha beta. Biochemical analyses of the CD4-CD8- T lymphocytes revealed three distinct TcR gamma delta; all are characterized by a 40 kDa delta chain but differed in their gamma chains. One gamma chain with a molecular mass of 38 kDaR is preferentially expressed on CD4-CD8- T lymphocytes derived from peripheral blood; another chain with molecular mass of 37 kDaR is evenly distributed between CD4-CD8- T lymphocytes from blood and lymphoid tissues.(ABSTRACT TRUNCATED AT 250 WORDS)
迄今为止，已描述了与CD3相关的T细胞受体（TcR）的两个亚类，它们由α链和β链（TcRαβ）或γ链和δ链（TcRγδ）组成。在这两个亚类中，TcRαβ是主要表达于人和啮齿动物外周T淋巴细胞上的亚类。TcRγδT淋巴细胞在这些物种中仅占一小部分。在迄今为止研究的所有哺乳动物物种中，猪的T淋巴细胞群体组成最为多样化，其特征在于表达CD4和CD8分化抗原。除了CD4 + CD8 - 和CD4 - CD8 + T淋巴细胞外，CD4 + CD8 + 和CD4 - CD8 - T淋巴细胞在胸腺外T淋巴细胞区室中也很突出。由于缺乏特异性单克隆抗体（mAb），迄今为止猪的TcR只能用生化和分子生物学方法进行表征。具有CD4 + 和/或CD8 + 表型的猪外周血T淋巴细胞上的TcR被表征为46 - 48 kDaR异二聚体，被认为代表猪的TcRαβ。对CD4 - CD8 - T淋巴细胞的生化分析揭示了三种不同的TcRγδ；它们都以40 kDa的δ链为特征，但γ链不同。一种分子量为38 kDaR的γ链优先表达于来自外周血的CD4 - CD8 - T淋巴细胞上；另一种分子量为37 kDaR的链均匀分布于来自血液和淋巴组织的CD4 - CD8 - T淋巴细胞之间。（摘要截短于250字）
