Davison A J, Davison M D
MRC Virology Unit, Institute of Virology, Glasgow, United Kingdom.
Virology. 1995 Feb 1;206(2):1035-43. doi: 10.1006/viro.1995.1026.
Emerging methods that couple mass spectrometry of fragments generated from isolated proteins with database searching offer a powerful means of identifying proteins and the genes that encode them. We have applied this technology to a herpesvirus, channel catfish virus, and have identified 12 genes, 11 viral and 1 cellular, that encode 16 principal structural proteins. These proteins include three components of the mature capsid and a potential scaffolding protein present in immature capsids, three protein kinases, a C3HC4 zinc-binding protein and cellular actin located in the tegument, and a multiply hydrophobic protein associated with the envelope.
将从分离蛋白质产生的片段进行质谱分析与数据库搜索相结合的新兴方法，为鉴定蛋白质及其编码基因提供了一种强大的手段。我们已将此技术应用于一种疱疹病毒——斑点叉尾鮰病毒，并鉴定出12个基因，其中11个是病毒基因，1个是细胞基因，这些基因编码16种主要结构蛋白。这些蛋白质包括成熟衣壳的三个组分和未成熟衣壳中存在的一种潜在支架蛋白、三种蛋白激酶、一种C3HC4锌结合蛋白以及位于被膜中的细胞肌动蛋白，还有一种与包膜相关的多重疏水蛋白。
