Schmitz J B, Albright A G, Kinchington P R, Jenkins F J
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814.
Virology. 1995 Feb 1;206(2):1055-65. doi: 10.1006/viro.1995.1028.
The herpes simplex virus type 1 (HSV-1) UL37 open reading frame encodes a 120-kDa late (gamma 1) phosphoprotein in infected cells. Analysis of isolated mature HSV virions and light particles revealed that the UL37 protein is a component of the virion. Detergent solubilization and protease digestion experiments suggest that the UL37 protein is part of the tegument structure. Indirect immunofluorescence experiments using HSV-1-infected cells and cells infected with a vaccinia recombinant virus that expresses the UL37 gene demonstrated that the UL37 protein is present in both the nucleus and cytoplasm of infected cells and that localization to the nucleus does not require additional HSV proteins.
单纯疱疹病毒1型（HSV-1）的UL37开放阅读框在受感染细胞中编码一种120 kDa的晚期（γ1）磷蛋白。对分离出的成熟HSV病毒粒子和轻粒子的分析表明，UL37蛋白是病毒粒子的一个组成部分。去污剂溶解和蛋白酶消化实验表明，UL37蛋白是包膜结构的一部分。使用HSV-1感染细胞和感染了表达UL37基因的痘苗重组病毒的细胞进行的间接免疫荧光实验表明，UL37蛋白存在于受感染细胞的细胞核和细胞质中，并且定位于细胞核不需要其他HSV蛋白。
