Volkova M O, Kostiukova N N, Kvetnaia A S
Zh Mikrobiol Epidemiol Immunobiol. 1994 Aug-Sep;Suppl 1:118-22.
The hyaluronidase activity of pneumococcal strains isolated from 39 patients with purulent meningitis, 26 patients with acute internal otitis and 130 healthy carriers was studied. All strains isolated from patients with purulent meningitis and meningoencephalitis and 84.6% of strains isolated in cases of otitis were found to have hyaluronidase, while in healthy children hyaluronidase-synthesizing strains were detected only in 11.5% of cases. Hyaluronidase titers in pneumococcal strains isolated from patients with meningitis and meningoencephalitis were significantly higher than in strains causing purulent otitis. At the same time strains with high hyaluronidase titers were also isolated from 7% of healthy carriers. Noncapsular pneumococcal strains had no hyaluronidase. The average value of the reverse correlation between hyaluronidase activity and the virulence of strains for mice was established. Hyaluronidase activity did not correlate with different serotypes of pneumococcal strains. The intranasal administration of hyaluronidase in high titers (> or = 1:8) to mice and rats led to the penetration of the infective agent through the hematoencephalic barrier of the animals and to their death from generalized pneumococcal infection. The role of hyaluronidase as the invasiveness factor of pneumococci was confirmed in experiments on the intranasal infection of mice with low active strains mixed with the preparation of exogenous hyaluronidase.
对从39例化脓性脑膜炎患者、26例急性中耳炎患者及130名健康带菌者分离出的肺炎球菌菌株的透明质酸酶活性进行了研究。从化脓性脑膜炎和脑膜脑炎患者分离出的所有菌株以及84.6%从中耳炎病例中分离出的菌株均被发现具有透明质酸酶，而在健康儿童中，仅在11.5%的病例中检测到合成透明质酸酶的菌株。从脑膜炎和脑膜脑炎患者分离出的肺炎球菌菌株中的透明质酸酶滴度显著高于引起化脓性中耳炎的菌株。同时，也从7%的健康带菌者中分离出了透明质酸酶滴度高的菌株。非荚膜肺炎球菌菌株没有透明质酸酶。确定了透明质酸酶活性与菌株对小鼠毒力之间的平均负相关值。透明质酸酶活性与肺炎球菌菌株的不同血清型无关。向小鼠和大鼠鼻内高滴度（≥1:8）施用透明质酸酶导致感染因子穿透动物的血脑屏障，并导致它们死于全身性肺炎球菌感染。在用低活性菌株与外源性透明质酸酶制剂混合对小鼠进行鼻内感染的实验中，证实了透明质酸酶作为肺炎球菌侵袭因子的作用。
