Rakowska M, Wojtczak L
Nencki Institute of Experimental Biology, Warsaw, Poland.
Biochem Biophys Res Commun. 1995 Feb 6;207(1):300-5. doi: 10.1006/bbrc.1995.1187.
Incorporation of [14C]serine into phosphatidylserine by the base exchange reaction in Ehrlich ascites tumor cells is inhibited by 30% by glucose and by 60% by 2-deoxyglucose (10 mM each). The inhibition by thapsigargin (0.2 microM) amounts to 80%. This inhibition is interpreted as being due to depletion of calcium stores in the endoplasmic reticulum and is compatible with the previous observation [Teplova, V. V., Bogucka, K., Czyz, A., Evtodienko, Yu.V., Duszyński, J., and Wojtczak, L. (1993) Biochem. Biophys. Res. Commun. 196, 1148-1154] that glucose and deoxyglucose elicit an increase of cytoplasmic [Ca2+] at the expense of intracellular Ca2+ stores and with the finding [Barańska, J. (1989) FEBS Lett. 256, 33-37] that the base exchange reaction requires high concentration of Ca2+ within the endoplasmic reticulum lumen.
在艾氏腹水瘤细胞中，通过碱基交换反应将[14C]丝氨酸掺入磷脂酰丝氨酸的过程，分别被葡萄糖抑制30%，被2-脱氧葡萄糖（各10 mM）抑制60%。毒胡萝卜素（0.2 microM）的抑制率达80%。这种抑制被解释为是由于内质网中钙储存的耗尽，并且与之前的观察结果[Teplova, V. V., Bogucka, K., Czyz, A., Evtodienko, Yu.V., Duszyński, J., and Wojtczak, L. (1993) Biochem. Biophys. Res. Commun. 196, 1148 - 1154]一致，即葡萄糖和脱氧葡萄糖以细胞内钙储存为代价引起细胞质[Ca2+]增加，也与[Barańska, J. (1989) FEBS Lett. 256, 33 - 37]的发现一致，即碱基交换反应需要内质网腔中高浓度的Ca2+。
