Teplova V V, Bogucka K, Czyz A, Evtodienko Y V, Duszyński J, Wojtczak L
Nencki Institute of Experimental Biology, Warsaw, Poland.
Biochem Biophys Res Commun. 1993 Nov 15;196(3):1148-54. doi: 10.1006/bbrc.1993.2371.
Concentration of free cytoplasmic Ca2+ ([Ca2+]i) in Ehrlich ascites tumor cells, measured using fura-2, amounted to 170-300 nM and was increased by 50-160 nM after addition of 10 mM D-glucose or D-2-deoxyglucose but not 3-O-methylglucose at pH 7.4. In the range of external pH between 6.8 and 7.8 the increase was higher at higher pH. This increase occurred within 30-60 s after addition of hexose and lasted for at least 10 min. This [Ca2+]i rise was observed both in presence and virtual absence of Ca2+ in the external medium. Pretreatment of the cells with thapsigargin resulted in a much smaller [Ca2+]i increase after addition of glucose or deoxyglucose. The mechanism of [Ca2+] in the external medium. Pretreatment of the cells with thapsigargin resulted in a much smaller [Ca2+]i increase after addition of glucose or deoxyglucose. The mechanism of [Ca2+]i rise evoked by glucose and deoxyglucose and its importance in switching cell metabolism from oxidative to glycolytic are discussed.
使用fura - 2测量艾氏腹水瘤细胞中游离细胞质Ca2 +（[Ca2 +]i）的浓度，在pH 7.4时为170 - 300 nM，添加10 mM D - 葡萄糖或D - 2 - 脱氧葡萄糖后增加50 - 160 nM，但添加3 - O - 甲基葡萄糖后无增加。在外部pH值6.8至7.8范围内，pH值越高增加幅度越大。这种增加在添加己糖后30 - 60秒内发生，并持续至少10分钟。在外部培养基存在和几乎不存在Ca2 +的情况下均观察到这种[Ca2 +]i升高。用毒胡萝卜素预处理细胞后，添加葡萄糖或脱氧葡萄糖后[Ca2 +]i的增加要小得多。讨论了葡萄糖和脱氧葡萄糖引起的[Ca2 +]i升高的机制及其在将细胞代谢从氧化转变为糖酵解中的重要性。
