de Micco C, Zoro P, Henry J F
INSERM U38, Faculté de Médecine, CHU Nord, Marseille.
Ann Pathol. 1994;14(6):378-83.
The enzyme thyroid-peroxidase (TPO) possesses in thyroid malignancy an immunological alteration disclosed by an anti-TPO monoclonal antibody termed MoAb47: a negative immunostaining with MoAb47 thus represent a marker of malignancy for thyroid tumors. The present work has been intended to investigate the ability of TPO immunodetection to assist the diagnosis of malignancy in fine-needle aspirates (FNA) of thyroid nodules. The study concerns 300 patients with histologically proven thyroid nodules. The results of TPO immunodetection have been compared to the final histological diagnosis and in 279 cases, to the results of standard cytological analysis. From 248 benign nodules, 215 yielded more than 80% positive cells. All malignant nodules had less than 80% positive cells and most of them less than 40%. According to these results, with 80% positive cells as the threshold between benign and suspicious or malignant results, the sensitivity of the method for the screening of malignancy is 100%, its specificity 86.7% and its overall accuracy 89%.
甲状腺过氧化物酶（TPO）在甲状腺恶性肿瘤中存在一种免疫改变，可被一种名为MoAb47的抗TPO单克隆抗体检测到：因此，用MoAb47进行阴性免疫染色是甲状腺肿瘤恶性的标志物。本研究旨在探讨TPO免疫检测在甲状腺结节细针穿刺抽吸物（FNA）中辅助诊断恶性肿瘤的能力。该研究涉及300例经组织学证实的甲状腺结节患者。TPO免疫检测结果与最终组织学诊断进行了比较，在279例病例中，还与标准细胞学分析结果进行了比较。在248个良性结节中，215个产生了超过80%的阳性细胞。所有恶性结节的阳性细胞均少于80%，其中大多数少于40%。根据这些结果，以80%阳性细胞作为良性与可疑或恶性结果的阈值，该方法筛查恶性肿瘤的敏感性为100%，特异性为86.7%，总体准确率为89%。
