Sauer N, Baier K, Gahrtz M, Stadler R, Stolz J, Truernit E
Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Germany.
Plant Mol Biol. 1994 Dec;26(5):1671-9. doi: 10.1007/BF00016496.
The fluxes of carbohydrates across the plasma membranes of higher-plant cells are catalysed mainly by monosaccharide and disaccharide-H+ symporters. cDNAs encoding these different transporters have been cloned recently and the functions and properties of the encoded proteins have been studied extensively in heterologous expression systems. Several of the proteins have been identified biochemically in these expression systems and their location in plants has been shown immunohistochemically or with transgenic plants which were transformed with reporter genes, expressed under the control of the promoters of individual transporter genes. In this paper we summarize the current knowledge on the molecular biology and biochemistry of higher-plant sugar transport proteins.
高等植物细胞碳水化合物跨质膜的通量主要由单糖和二糖-H⁺同向转运体催化。最近已克隆出编码这些不同转运体的cDNA,并在异源表达系统中广泛研究了所编码蛋白质的功能和特性。在这些表达系统中已通过生化方法鉴定出几种蛋白质,并且通过免疫组织化学或利用用报告基因转化的转基因植物(在单个转运体基因启动子的控制下表达)显示了它们在植物中的定位。在本文中,我们总结了目前关于高等植物糖转运蛋白分子生物学和生物化学的知识。