Sabry I, Moussa F I, el-Toweissy M Y, Shabana M B
Zoology Department, Faculty of Science, Alexandria University, Egypt.
Endocr Regul. 1994 Sep;28(3):133-40.
The exposure of juvenile Clarias lazera to acute (pH 3.1 for 96 h) and chronic (pH 5.4 or 4.7 for 12 weeks) acid stress (H2SO4) resulted in a number of physiological responses which could be considered as adaptive to the effects of stress. These responses were evident in the rapid rise of serum cortisol level from 43.0 +/- 0.76 microgram/dl in the control to 71.0 +/- 1.06 micrograms/dl (P < 0.001) 24 h after acute exposure. Also serum glucose level was increased from 123.66 +/- 1.85 mg/dl in the control and reached 295.0 +/- 2.89 mg/dl (P < 0.001) by the end of the experimental period (96 h). Chronic exposure of the fish to the two levels of pH (5.4 and 4.7) caused significant increase in serum cortisol and serum glucose level starting from the first week of exposure and lasted throughout the study period (12 weeks). As to the liver glycogen content, a slight but significant drop was found from 11.06 +/- 0.03 mg/100 mg tissue dry weight in the control to 10.80 +/- 0.06 mg/100 mg tissue dry weight (P < 0.025) 24 h after acute acid exposure. However, the values at 72 and 96 h were significantly higher than those in the control. In the chronic exposure study, the fish maintained at pH 5.4 showed a marked increase in liver glycogen content starting from week 2 and lasted till the end of experimental period (12 weeks). As early as after one week of treatment, in fish maintained in pH 4.7, liver glycogen content surged and remained significantly high till the twelfth week.
将幼年革胡子鲶暴露于急性（pH 3.1，持续96小时）和慢性（pH 5.4或4.7，持续12周）酸胁迫（硫酸）下，会引发一系列生理反应，这些反应可被视为对胁迫影响的适应性反应。急性暴露24小时后，血清皮质醇水平从对照组的43.0±0.76微克/分升迅速升至71.0±1.06微克/分升（P<0.001），这些反应很明显。此外，血清葡萄糖水平从对照组的123.66±1.85毫克/分升开始上升，到实验期结束时（96小时）达到295.0±2.89毫克/分升（P<0.001）。将鱼长期暴露于两种pH水平（5.4和4.7）下，从暴露的第一周开始，血清皮质醇和血清葡萄糖水平就显著升高，并持续整个研究期（12周）。至于肝脏糖原含量，急性酸暴露24小时后，从对照组的11.06±0.03毫克/100毫克组织干重略有但显著下降至10.80±0.06毫克/100毫克组织干重（P<0.025）。然而，72小时和96小时的值显著高于对照组。在慢性暴露研究中，维持在pH 5.4的鱼从第2周开始肝脏糖原含量显著增加，并持续到实验期结束（12周）。早在处理一周后，维持在pH 4.7的鱼肝脏糖原含量就激增，并一直保持到第12周显著高水平。
