Mills P J, Dimsdale J E, Nelesen R A, Ziegler M G
Department of Psychiatry, UCSD Medical Center, University of California.
Clin Exp Hypertens. 1994 Nov;16(6):825-33. doi: 10.3109/10641969409078028.
While the importance of receptor-mediated intracellular cyclic AMP in blood pressure regulation is well documented, few studies have evaluated the physiologic relevance of cyclic AMP exported from cells. We report evidence of a relationship between blood pressure and the transport of intracellular cyclic AMP from lymphocytes. Twenty-eight hypertensive and 56 normotensive white and black volunteers (mean age 40 years) were studied. Both intra- and extracellular concentrations of cyclic AMP were determined in lymphocytes following incubation with 10(-5) M isoproterenol. Compared to normotensives, hypertensives (p = 0.001), particularly white hypertensives (p = 0.023) had higher levels of exported cyclic AMP. These values were independent of intracellular concentrations of cyclic AMP, which were similar across the groups. Exported cyclic AMP was independent of both sodium excretion and beta-adrenergic receptor sensitivity, the latter being lower in white hypertensives (p = 0.024). Across all subjects, exported cyclic AMP was correlated with MAP (r = .39, p < 0.001). These findings indicate that the active transport of cyclic AMP may be enhanced in hypertension and suggest a possible pathway which might explain existing data of increased cyclic AMP levels in hypertension.
虽然受体介导的细胞内环磷酸腺苷（cAMP）在血压调节中的重要性已有充分记录，但很少有研究评估细胞分泌的cAMP的生理相关性。我们报告了血压与淋巴细胞内cAMP转运之间关系的证据。对28名高血压患者和56名血压正常的白种人和黑种人志愿者（平均年龄40岁）进行了研究。在用10^(-5)M异丙肾上腺素孵育后，测定淋巴细胞内和细胞外的cAMP浓度。与血压正常者相比，高血压患者（p = 0.001），尤其是白人高血压患者（p = 0.023）的cAMP分泌水平更高。这些值与细胞内cAMP浓度无关，各组细胞内cAMP浓度相似。cAMP的分泌与钠排泄和β-肾上腺素能受体敏感性均无关，后者在白人高血压患者中较低（p = 0.024）。在所有受试者中，cAMP的分泌与平均动脉压（MAP）相关（r = 0.39，p < 0.001）。这些发现表明，高血压患者中cAMP的主动转运可能增强，这提示了一条可能的途径，或许可以解释高血压患者中cAMP水平升高的现有数据。
